Anti-POLR2J Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52435

Core Product Specifications and Parameters

Biological Background: POLR2J Function and Localization

• Identity: POLR2J (also designated RPB11, RPB11A, RPB11m, hRPB14) is the DNA-directed RNA polymerase II subunit RPB11-a, encoded by the POLR2J gene (GeneID: 5439). It is also known as RNA polymerase II 13.3 kDa subunit.
• Core function: As a core component of RNA polymerase II (Pol II), POLR2J contributes to the DNA-dependent synthesis of mRNA precursors and many functional non-coding RNAs using ribonucleoside triphosphates as substrates.
• Subcellular localization: Predominantly localized to the nucleus, consistent with its essential role in transcription.
• Tissue expression: Ubiquitously expressed in human tissues, with particularly high levels detected in heart and skeletal muscle.
• Post-translational modifications: Reported to undergo acetylation; direct protein sequencing and proteomics identification have been performed.
• Structural context: A 3D-structure of the Pol II complex is available, and POLR2J is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the near full-length human POLR2J (residues 1-117). For Western blotting, expect a band at approximately 13 kDa, consistent with the predicted molecular weight; use appropriate gel percentage for low-molecular-weight proteins.
• IF/ICC applications should reveal a nuclear staining pattern due to the protein's nuclear localization; validate staining specificity in your cell type.
• Cross-reactivity is reported for human and mouse; when working with mouse models, verify performance in the relevant sample system.
• The antibody is supplied as a rabbit polyclonal; consider lot-to-lot consistency and validate for your specific analytical requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials and technical services—from concept to clinic. This rabbit polyclonal antibody against POLR2J serves as a reliable tool for investigating RNA polymerase II transcription mechanisms, with cross-reactivity to mouse broadening its utility. Our sourcing capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials, all designed to accelerate your assay development and research workflows.