Anti-POLR2H Polyclonal Antibody for WB, IP, ELISA - P52434

Core Product Specifications and Parameters

Biological Background: POLR2H Function and Localization

• POLR2H encodes the DNA-directed RNA polymerases I, II, and III subunit RPABC3, a common component of all three nuclear RNA polymerases, essential for transcription of rRNA precursors (Pol I), mRNA precursors and non-coding RNAs (Pol II), and small RNAs like 5S rRNA and tRNAs (Pol III).
• The protein is also known as RPB8, RPB17, and hRPB8 homolog, functioning as a core subunit within the polymerase complexes that catalyze DNA-templated RNA synthesis.
• POLR2H binds DNA and participates directly in transcription as a DNA-directed RNA polymerase, playing a structural role in the polymerase active center.
• Subcellular localization is exclusively nuclear, with enrichment in the nucleolus where Pol I transcription occurs, and distributed throughout the nucleoplasm for Pol II and Pol III activities.
• Post-translational modifications include acetylation, and multiple isoforms are produced by alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length protein (aa1-150) expressed as a recombinant fusion, favoring recognition of native conformations; consider verifying reactivity under denaturing vs. native conditions for each application.
• For Western blotting, a band near 17 kDa is expected; include appropriate positive (e.g., HeLa, HEK293) and negative controls to confirm specificity.
• In immunoprecipitation, the polyclonal nature may enhance capture efficiency but also increase background; pre-clearing lysates and using isotype controls are recommended.
• ELISA performance depends on coating antigen format; the recombinant protein immunogen serves as a positive control for assay development.
• Cross-reactivity is reported for human, mouse, and rat; validate in your specific species and tissue of interest, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable access to IVD-grade raw materials. This anti-POLR2H antibody is suitable for transcription research and RNA polymerase studies, and can be sourced alongside validated antibody pairs, bulk ancillary reagents, and rare target raw materials. For customized procurement or technical consultation, CamelBio connects your project from concept to clinic with a comprehensive supply chain.