Anti-POLR2H Polyclonal Antibody for WB, IP, ELISA - P52434

Core Product Specifications and Parameters

Biological Background: POLR2H Function and Localization

• POLR2H encodes the DNA-directed RNA polymerases I, II, and III subunit RPABC3, also known as RNA polymerase II subunit H (RPB8) and RPB17.
• This subunit is a shared component of RNA polymerases I, II, and III, which catalyze the transcription of DNA into ribosomal RNA precursors, mRNA precursors, and small functional RNAs (e.g., 5S rRNA and tRNAs).
• POLR2H localizes to the nucleus and nucleolus, aligning with its function in transcription.
• Post-translational modifications include acetylation, and alternative splicing yields multiple isoforms.
• The calculated molecular weight is approximately 17.1 kDa, matching the observed ~17 kDa.
• As part of the transcription machinery, POLR2H participates in DNA binding and RNA synthesis.
• Protein identity has been confirmed by direct protein sequencing and proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–150 of human POLR2H; the full-length protein is expected at ~17 kDa in Western blot.
• Validate reactivity in your specific human or mouse sample system, as polyclonal antibodies can exhibit lot-to-lot variability.
• For Western blot, consider loading a nuclear or whole-cell extract positive control and appropriate normalization.
• Immunoprecipitation conditions (antibody-to-lysate ratio, incubation time) should be optimized; a pre-clearing step may reduce non-specific binding.
• For ELISA, this antibody can serve as a capture or detection reagent when paired with another POLR2H-specific antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a streamlined supply channel for IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-POLR2H. For projects targeting transcription-related biomarkers, we offer bulk reagents, custom production services, and sourcing of rare targets—all designed to bridge your concept to clinical application with efficiency and reliability.