Anti-POLR2G Rabbit Polyclonal Antibody for WB, ELISA - P62487

Core Product Specifications and Parameters

Biological Background: POLR2G Function and Localization

• DNA-directed RNA polymerase II subunit RPB7 (POLR2G), also known as RNA polymerase II 19 kDa subunit (RPB19) or RPB7, is a core subunit of RNA polymerase II (Pol II) that synthesizes mRNA precursors and functional non-coding RNAs.
• POLR2G forms a subcomplex with the RPB4 subunit (POLR2D), which binds to a pocket formed by the RPB1, RPB2, and RPB6 subunits at the base of the clamp element of Pol II.
• The RPB4-RPB7 subcomplex locks the clamp in a closed conformation via RPB7, preventing double-stranded DNA from entering the active site cleft prematurely.
• In addition to its structural role, the POLR2G/RPB7 subcomplex has an intrinsic ability to bind single-stranded DNA and RNA, supporting its function in transcription elongation.
• POLR2G belongs to the eukaryotic RNA polymerase II subunit family, with conserved structural and functional roles across species.
• The protein localizes exclusively to the nucleus, consistent with its essential role in nuclear transcription.
• Key structural and functional features of POLR2G have been elucidated by 3D-structure determination, highlighting its importance in the Pol II machinery.
• Known post-translational modifications and proteomics identification studies underscore its regulation and integration into the transcription apparatus.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human POLR2G protein (amino acids 1–172), which may maximize antibody recognition across native and denatured conformations; suitable for both WB and ELISA applications.
• For Western blot analysis, consider using whole-cell lysates from human, mouse, or rat origin, and expect a band around 19 kDa; loading controls should be selected accordingly.
• For ELISA, consider titrating the antibody against recombinant or native POLR2G antigen; cross-reactivity with mouse and rat orthologs may be exploited for multi-species studies.
• As a polyclonal antibody, lot-to-lot variability can occur; validation of each new batch in the specific assay system is recommended to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

As a leading one-stop IVD raw-material partner, CamelBio supplies high-quality antibodies like this anti-POLR2G antibody to accelerate your RNA polymerase II and transcription studies. In addition to validated antibody pairs and optimized monoclonal/polyclonal antibodies, CamelBio offers bulk ancillary reagents and rare target sourcing to support your diagnostic and research needs from concept to clinic.