Anti-POLR2F Polyclonal Antibody for WB, ELISA - P61218

Core Product Specifications and Parameters

Biological Background: POLR2F Function and Localization

• DNA-directed RNA polymerases I, II, and III subunit RPABC2, also known as POLR2F, RPB6, RPC15, and RPABC14.4, is a common subunit shared by all three nuclear RNA polymerases.
• As a component of RNA polymerases I, II, and III, it participates in the synthesis of ribosomal RNA precursors, messenger RNA precursors, various non-coding RNAs, 5S rRNA, and transfer RNAs.
• Within RNA polymerase II, POLR2F is part of the clamp element and, together with portions of POLR2A/RPB1 and POLR2B/RPB2, forms a binding pocket for the POLR2D/RPB4-POLR2G/RPB7 subcomplex, contributing to polymerase stability and processivity.
• POLR2F localizes to the nucleus and is also found in the nucleolus, consistent with its role in multiple transcription systems.
• The protein is subject to post-translational modifications, including acetylation and phosphorylation, which may regulate its function or interactions.
• Structural studies have resolved the three-dimensional architecture of POLR2F within the polymerase complex, providing insights into its positioning and roles.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length POLR2F sequence (amino acids 1-127), so the antibody is likely to recognize both native and denatured protein; for Western blot, a band near 14 kDa is expected.
• When performing WB, validate antibody specificity using appropriate controls (e.g., siRNA knockdown or knockout cell lysates) and include loading controls such as GAPDH or β-actin.
• For ELISA applications, optimize coating conditions and consider using the antibody as either a capture or detection reagent, depending on assay design.
• Although cross-reactivity is indicated for human and rat, verify performance in your specific sample matrix due to potential species-specific variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, offering researchers and manufacturers access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target acquisition. This anti-POLR2F antibody is well-suited for transcription-related applications, and our team can assist with scaling, technical consultation, and supply chain management to accelerate your diagnostic development from concept to clinic.