Anti-POLR2D Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O15514

Core Product Specifications and Parameters

Biological Background: POLR2D Function and Localization

• POLR2D (DNA-directed RNA polymerase II subunit RPB4, also known as RBP4, RPB16) is a core subunit of human RNA polymerase II (Pol II), the central enzyme responsible for synthesizing mRNA precursors and many non-coding RNAs.
• As part of a conserved subcomplex with POLR2G/RPB7, POLR2D binds to a pocket formed by the RPB1, RPB2, and RPABC2 subunits near the base of the Pol II clamp element, helping to lock the clamp in a closed conformation and preventing double-stranded DNA from entering the active site cleft prematurely.
• The POLR2D/POLR2G subcomplex also interacts with single-stranded DNA and RNA, suggesting roles in nucleic acid handling during transcription initiation or elongation.
• POLR2D is localized exclusively in the nucleus, consistent with its role in the transcription machinery.
• The protein is subject to structural studies (keyword: 3D-structure) and has been identified by mass spectrometry-based proteomics, supporting its stable incorporation into Pol II.
• No tissue-specific isoforms or post-translational modifications have been reported for POLR2D, implying a relatively uniform role across cell types.

Experimental Guidance and Technical Tips

• The immunogen consists of recombinant protein spanning amino acids 1–142 of human POLR2D. This N-terminal region is well-conserved, which may explain the antibody's cross-reactivity with mouse and rat.
• For Western blot (WB), the predicted molecular weight of POLR2D is approximately 16 kDa; use a high-percentage gel or gradient gel to ensure clear separation of low-molecular-weight bands and include appropriate loading controls.
• In IHC-P, strong nuclear staining is expected; consider antigen retrieval protocols and validate specificity using blocking peptides or knockdown/knockout control tissues where possible.
• For ELISA, the antibody can be used for direct or sandwich assays; however, end-users should optimize coating conditions and antibody concentrations based on their specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive IVD raw material supply, from concept to clinic. This anti-POLR2D monoclonal antibody is part of our expanding catalog for transcription and nuclear-protein targets. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets, helping you streamline assay development and production.