Anti-POLR2D Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O15514

Core Product Specifications and Parameters

Biological Background: POLR2D Function and Localization

• POLR2D, also known as RPB4, RPB16, or DNA-directed RNA polymerase II subunit D, is a core subunit of RNA polymerase II (Pol II), responsible for synthesizing mRNA precursors and non-coding RNAs.
• It forms a stable subcomplex with POLR2G/RPB7 that binds to a specific pocket formed by the large subunits RPB1, RPB2, and RPB6 at the base of the Pol II clamp element.
• The POLR2D/RPB4-POLR2G/RPB7 subcomplex locks the clamp in a closed conformation via POLR2G/RPB7, preventing double-stranded DNA from entering the active site cleft and thereby regulating transcription initiation.
• This subcomplex also possesses single-stranded DNA and RNA binding activity, contributing to polymerase processivity and transcript stabilization.
• POLR2D localizes exclusively to the nucleus, consistent with its role in DNA-templated transcription.
• According to UniProt, this protein is part of the reference proteome and has been identified through proteomics identification and 3D structural studies.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing amino acids 1-142, which corresponds to the full-length POLR2D sequence. This design should provide high specificity for the native protein.
• Given the high sequence identity between human and mouse POLR2D, the antibody is expected to cross-react reliably in mouse samples, as supported by the supplier's validation.
• Western blot (WB) detection should yield a prominent band at approximately 16 kDa; use appropriate molecular weight markers for size confirmation.
• For immunofluorescence (IF/ICC), anticipate exclusive nuclear staining; counterstain with DAPI to confirm colocalization.
• In immunoprecipitation (IP) experiments, the antibody may pull down the POLR2D–POLR2G subcomplex, enabling analysis of Pol II subunit interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for sourcing high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This anti-POLR2D polyclonal antibody is an excellent tool for studying RNA polymerase II-mediated transcription, chromatin dynamics, and gene regulation. Our supply chain capabilities extend to bulk antibody orders, customized immunogen production, and rare target sourcing, ensuring seamless support from concept to clinic.