Anti-POLR2B Polyclonal Antibody for WB, IHC-P, ELISA - P30876

Core Product Specifications and Parameters

Biological Background: POLR2B Function and Localization

• POLR2B (RNA polymerase II subunit B, RPB2) is the second largest subunit of RNA polymerase II (Pol II), the enzyme that synthesizes mRNA precursors and numerous non‑coding RNAs in a DNA‑dependent manner. Related references: PMID:27193682 PMID:30190596 PMID:9852112
• Together with the largest subunit POLR2A/RPB1, POLR2B forms the catalytic center, coordinating two Mg²⁺ ions and providing lysine residues that facilitate Watson–Crick base pairing during nucleotide addition. Related references: PMID:30190596 PMID:9852112
• POLR2B possesses an intrinsic 3′→5′ exonuclease proofreading activity that corrects misincorporated ribonucleotides by backtracking the nascent RNA and cleaving the phosphodiester bond. Related references: PMID:8381534
• Beyond DNA‑dependent transcription, POLR2B catalyzes RNA‑directed RNA polymerisation, extending the 3′‑end of certain non‑coding RNAs using an internal template, which may reduce the stability of repressive ncRNAs.
• The protein localises exclusively to the nucleus, consistent with its transcriptional role.
• POLR2B is subject to regulatory phosphorylation and methylation and contains zinc‑finger motifs that likely contribute to its structural integrity and interactions with nucleic acids.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 260 amino acids of human POLR2B. This region is suitable for detecting the full‑length ~134 kDa protein in western blot; confirm specificity using knockdown or knockout controls.
• For immunohistochemistry (IHC‑P), consider optimizing antigen retrieval and primary antibody dilution, as formalin‑fixed paraffin‑embedded tissues may require different conditions.
• This antibody recognizes POLR2B from human, mouse, and rat; validate in the relevant sample system when moving between species.
• In ELISA, use the antibody as a capture or detection reagent according to the assay format; cross‑check with a known standard.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner, offering diagnostic developers and laboratories a single entry point for high‑quality antibodies, antigen pairs, and ancillary reagents. For transcription‑focused projects, we supply validated anti‑POLR2B products and can assist with sourcing rare‑target raw materials to support your R&D pipeline from feasibility to commercialisation.