Anti-POLR1E Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9GZS1

Core Product Specifications and Parameters

Biological Background: POLR1E Function and Localization

• POLR1E, also designated as PAF53, PRAF1, or RPA49, is a subunit of the DNA-directed RNA polymerase I (Pol I) complex, which is essential for the synthesis of ribosomal RNA (rRNA) precursors from ribosomal DNA templates.
• Functioning within the nucleolus, POLR1E mediates the interaction between Pol I and the upstream binding transcription factor UBTF/UBF, a key step in forming the transcription initiation complex at the ribosomal DNA promoter. Related references: PMID:24207024, PMID:34671025, PMID:34887565
• Consistent with its role in rRNA transcription, the protein localizes predominantly to the nucleolus.
• POLR1E is subject to regulatory post-translational modifications, including acetylation and phosphorylation, as indicated by proteomics studies.
• The POLR1E gene is known to undergo alternative splicing, which may generate multiple transcript variants; the canonical full-length isoform has a calculated molecular mass of approximately 47 kDa (47260 Da according to UniProt).
• The target sequence is conserved across human, mouse, and rat, which aligns with the reported cross-reactivity of this antibody.

Experimental Guidance and Technical Tips

• For Western blot analysis, a band corresponding to the full-length POLR1E protein is expected at around 47 kDa. Nuclear or nucleolar enriched lysates may improve signal detection.
• When using this antibody for immunoprecipitation (IP), consider validating the co-immunoprecipitation of other Pol I complex members to confirm functionality.
• In ELISA applications, use the recombinant immunogen (amino acids 1-419) as a positive control to assess antibody titer and specificity.
• Always include appropriate negative and loading controls, and verify expression in your particular cell line or tissue type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to empowering diagnostic developers and research laboratories with a comprehensive portfolio of IVD raw materials. Our catalog includes validated antibodies, antigens, and ancillary reagents for a wide range of targets, including transcription machinery components like POLR1E. Beyond standard products, we offer customized sourcing and technical consulting services to accelerate your assay development from concept to clinical application.