Anti-POLR1B Polyclonal Antibody for WB, ELISA - Q9H9Y6

Core Product Specifications and Parameters

Biological Background: POLR1B Function and Localization

• POLR1B (also known as RPA135, RPA2) is the second largest subunit of DNA-directed RNA polymerase I (Pol I), which is essential for the synthesis of ribosomal RNA precursors.
  Related references: PMID:11250903, PMID:11283244, PMID:16858408

• It forms the active center of Pol I together with the largest subunit POLR1A, coordinating two catalytic magnesium ions and providing lysine residues that facilitate Watson-Crick base pairing during nucleotide addition.
  Related references: PMID:11250903, PMID:11283244, PMID:16809778

• The enzyme transcribes 47S pre-rRNA from multicopy rRNA gene clusters, which is subsequently processed into 5.8S, 18S, and 28S ribosomal RNAs.
  Related references: PMID:11250903, PMID:16858408

• POLR1B possesses proofreading activity; the polymerase can pause and backtrack, allowing the cleavage of misincorporated nucleotides via the subunit POLR1H.
  Related references: PMID:11250903, PMID:11283244, PMID:16809778

• It localizes to the nucleolus and chromosomes and is highly processive, forming "Miller tree" structures during rDNA transcription.
  Related references: PMID:11250903, PMID:11283244, PMID:16858408

• UniProt keywords highlight that POLR1B is a phosphoprotein and contains zinc-finger domains, indicating roles in metal-binding and phosphorylation-mediated regulation.

• Alternative splicing generates distinct isoforms, and variants have been associated with disease, though specific pathologies are not detailed.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band around 128 kDa is expected; use a 6–8% SDS-PAGE gel for optimal resolution and validate detection in your specific human or mouse cell lysates.
• When performing ELISA, consider optimizing coating conditions and antibody dilutions using the recombinant immunogen as a standard to ensure robust signal detection.
• As a polyclonal antibody, it may recognize multiple epitopes; include appropriate negative controls to confirm specificity in your assay system.
• Store the antibody as recommended upon receipt (ice bag shipping suggests storage at -20°C or below) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, providing a one-stop sourcing solution from concept to clinic. For researchers focusing on RNA polymerase I and nucleolar biology, we offer a comprehensive portfolio including validated antibody pairs, optimized polyclonal antibodies like anti-POLR1B, bulk ancillary reagents, and sourcing for rare target proteins. Our commitment to quality supply helps accelerate your diagnostic and research assay development.