Anti-POLL Rabbit Polyclonal Antibody for WB, ELISA - Q9UGP5

Core Product Specifications and Parameters

Biological Background: POLL (DNA Polymerase Lambda) Function and Localization

• DNA polymerase lambda (POLL) is a DNA polymerase that plays a critical role in DNA repair, including base excision repair (BER), non-homologous end joining (NHEJ), and homologous recombination (HR). Related references: PMID:11457865, PMID:19806195, PMID:20693240, PMID:30250067
• It possesses both template-dependent DNA polymerase activity and template-independent terminal transferase activity, enabling it to fill short gaps and add nucleotides to DNA ends. Related references: PMID:10887191, PMID:10982892, PMID:12809503
• POLL also exhibits 5'-deoxyribose-5-phosphate (dRP) lyase activity, a key step in BER that removes the 5' sugar phosphate group from incised abasic sites. Related references: PMID:11457865, PMID:19806195
• It is predominantly localized in the nucleus and associates with chromosomes, consistent with its function in DNA repair and replication.
• POLL is abundantly expressed in the testis, but also found in other tissues, suggesting roles in both somatic and meiotic DNA repair.
• The protein is subject to alternative splicing, and keywords from UniProt highlight its involvement in DNA damage, DNA repair, DNA replication, and its metal-binding requirement (manganese).

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 421-575) of human POLL, encompassing the DNA polymerase catalytic domain; this region is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting (WB), a major band at approximately 63 kDa is expected; validate specificity using positive controls (e.g., HeLa, HEK293, or testis lysates) and consider blocking peptide or siRNA knockdown experiments.
• In ELISA applications, titrate the antibody against recombinant POLL protein or the immunogen peptide to determine optimal working dilutions.
• To study POLL's role in DNA repair, consider inducing DNA damage with agents such as bleomycin or etoposide, or synchronize cells to monitor expression changes throughout the cell cycle.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. We offer validated antibody pairs, optimized polyclonal antibodies like this anti-POLL rabbit pAb, and bulk ancillary reagents to streamline your DNA repair and genomic stability assay development. For rare targets or custom sourcing needs, CamelBio provides tailored procurement solutions to accelerate your project timelines.