Anti-POLG2 Rabbit Polyclonal Antibody - Q9UHN1

Core Product Specifications and Parameters

Biological Background: POLG2 Function and Localization

• POLG2 is the accessory subunit of DNA polymerase gamma, the sole polymerase responsible for mitochondrial DNA (mtDNA) replication.
• Also known as p55, MtPolB, or PolG-beta, it serves as an allosteric regulator of the holoenzyme, enhancing polymerase activity and processivity.
• POLG2 suppresses the exonucleolytic proofreading activity of the catalytic subunit POLG, particularly toward homopolymeric templates with mismatched termini.
• The protein localizes to the mitochondrial matrix and nucleoid, where mtDNA replication occurs.
• POLG2 binds to single-stranded DNA, facilitating the polymerase's interaction with the template.
• Mutations in POLG2 are associated with mitochondrial disorders, including progressive external ophthalmoplegia (PEOA4) and mitochondrial DNA depletion syndromes (MTDPS16).
• Keywords: DNA replication, DNA-binding, mitochondrion, phosphoprotein, primary mitochondrial disease, proteomics identification, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 216-485 of human POLG2, covering a region that may contribute to DNA binding or interaction with the catalytic subunit. Researchers should validate specificity in their experimental system.
• For Western blot, a band around 55kDa is expected in human, mouse, and rat samples. Positive controls such as HeLa or HepG2 cell lysates are recommended.
• In immunofluorescence and IHC-P, co-localization with mitochondrial markers (e.g., TOMM20, COX IV) can confirm specific mitochondrial staining.
• As a polyclonal antibody, potential batch-to-batch variability should be considered; pilot experiments are advised to optimize working dilutions for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our anti-POLG2 rabbit polyclonal antibody is ideal for mitochondrial DNA replication studies and related diagnostic applications. Beyond this product, we offer validated antibody pairs, bulk ancillary reagents, and customized rare target sourcing to streamline your IVD development.