Anti-POLE4 Rabbit Polyclonal Antibody for WB and ELISA - Q9NR33

Core Product Specifications and Parameters

Biological Background: DNA polymerase epsilon subunit 4 Function and Localization

• DNA polymerase epsilon subunit 4 (POLE4, also known as DNA polymerase II subunit 4 or p12) is an accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Related references: PMID:10801849
• It plays a role in DNA repair and chromosomal DNA replication, contributing to genome maintenance (By similarity).
• POLE4 localizes to the nucleus, where it functions within the replication fork.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or interactions.
• The protein is characterized by DNA-binding capability and is classified under reference proteome.
• Calculated molecular weight is approximately 12.2 kDa (UniProt), consistent with the observed 12 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100) of human POLE4. For western blot, consider testing a range of antibody dilutions to achieve optimal signal-to-noise ratio for the ~12 kDa band.
• Since the antibody cross-reacts with human and mouse POLE4, it can be used in both species. Validate the performance in the relevant tissue or cell line.
• For ELISA, determine the optimal coating antigen and antibody concentration by checkerboard titration to ensure sensitivity.
• Store the antibody under recommended conditions (shipped on ice bag) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. This anti-POLE4 antibody supports studies in DNA replication, repair, and chromatin biology. Beyond this product, CamelBio provides validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare-target raw materials to streamline your assay development and production.