Anti-POLE4 Polyclonal Antibody for WB, ELISA - Q9NR33

Core Product Specifications and Parameters

Biological Background: DNA polymerase epsilon subunit 4 Function and Localization

• POLE4, also known as DNA polymerase epsilon subunit p12 or DNA polymerase II subunit 4, is an accessory component of the DNA polymerase epsilon complex. Related references: PMID:10801849
• It participates in DNA repair and chromosomal DNA replication, but the exact mechanisms are inferred by similarity with other species.
• The protein is localized in the nucleus, as expected for a DNA replication/repair factor.
• POLE4 undergoes acetylation and phosphorylation, which are potential regulatory post-translational modifications.
• It is a DNA-binding protein, supporting its direct interaction with nucleic acids during replication and repair processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length POLE4 protein (1-117 aa), suggesting the antibody may recognize native and denatured forms; consider optimizing conditions for your specific assay.
• For WB, since POLE4 has a predicted molecular weight of ~12 kDa, ensure proper transfer and membrane pore size for small proteins.
• ELISA can be used for quantitative detection; validate with recombinant or native protein controls in your sample matrix.
• Specificity for human POLE4 is indicated; cross-reactivity with other species has not been tested, so verify if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement channel for IVD raw materials, including this high-quality anti-POLE4 polyclonal antibody. Beyond single antibodies, we supply validated antibody pairs, optimized hybridoma-derived monoclonals, and bulk ancillary reagents. For your specialized DNA replication and repair research needs, our team can source or develop rare-target raw materials to accelerate your assay development.