Anti-POLE2 Rabbit Polyclonal Antibody for WB and ELISA - P56282

Core Product Specifications and Parameters

Biological Background: POLE2 Function and Localization

• Encodes DNA polymerase epsilon subunit 2 (POLE2), also known as DNA polymerase II subunit 2 or DNA polymerase epsilon subunit B.
• Accessory component of the DNA polymerase epsilon complex; participates in chromosomal DNA replication and DNA repair (PubMed:10801849). Related references: PMID:10801849
• Belongs to the DNA polymerase epsilon family and is essential for leading strand synthesis during replication.
• Primarily localized to the nucleus, consistent with its role in genomic maintenance.
• Contains conserved DNA-binding domains crucial for processivity and interaction with the catalytic subunit.
• Undergoes alternative splicing, producing isoforms that may have distinct regulatory functions.
• Classified under keywords including DNA replication, DNA-binding, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–290 of human POLE2; consider this region when designing blocking peptides or epitope mapping.
• For Western blot, the expected band is approximately 60 kDa; optimize gel percentage and transfer conditions accordingly.
• Validate reactivity in your specific sample system; cross-reactivity is indicated for human and mouse.
• ELISA performance may depend on coating antigen and detection conditions; titration of the antibody is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to IVD raw materials, including validated polyclonal antibodies like anti-POLE2. For DNA replication and repair targets, we offer one-stop sourcing from concept to clinic, covering bulk reagents, rare target sourcing, and technical consultation to accelerate assay development.