Anti-POLD1 Monoclonal Antibody for WB, ELISA - P28340

Core Product Specifications and Parameters

Biological Background: POLD1 Function and Localization

• POLD1 (DNA polymerase delta catalytic subunit) is the core catalytic component of the trimeric (Pol-delta3) and tetrameric (Pol-delta4) DNA polymerase delta complexes, essential for high-fidelity genome replication including lagging strand synthesis and various DNA repair processes.
  Related references:
  PMID:16510448 | PMID:19074196 | PMID:20334433
• Exhibits dual enzymatic activities: DNA polymerase and 3ʹ-5ʹ exonuclease proofreading, both critical for maintaining genomic stability.
  Related references:
  PMID:16510448 | PMID:19074196 | PMID:20334433
• Requires accessory subunits POLD2, POLD3, and POLD4 for full activity; the presence or absence of POLD4 differentiates Pol-delta4 and Pol-delta3 complexes, with Pol-delta3 exhibiting higher proofreading activity and reduced strand displacement, making it ideally suited for Okazaki fragment processing.
  Related references:
  PMID:19074196 | PMID:20334433 | PMID:24035200
• Participates in nucleotide excision repair (NER) after UV damage, undertaking approximately half of repair synthesis together with DNA polymerase kappa.
  Related references:
  PMID:20227374
• Under replication stress, promotes break-induced replication (BIR) to repair broken forks, a process dependent on POLD3 and POLD4.
  Related references:
  PMID:24310611
• Mediates translesion synthesis (TLS) across damaged templates harboring O6-methylguanine, 8-oxoguanine, or abasic sites, contributing to tolerance of DNA lesions.
  Related references:
  PMID:19074196 | PMID:24191025
• Localizes to the nucleus, consistent with its roles in replication and repair.
• Widely expressed across tissues, with notably high expression in heart and lung, as indicated by tissue specificity data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from the C‑terminal region (amino acids 1000–1107) of human POLD1. For western blot (WB), consider using whole‑cell lysates from human, mouse, or rat tissues (e.g., heart or lung) where POLD1 expression is abundant, and expect a band near 124 kDa.
• For ELISA applications, validate the antibody using recombinant POLD1 protein or the immunizing peptide as a standard, and optimize coating and detection conditions for your specific assay format.
• Due to high sequence conservation across human, mouse, and rat, this antibody is predicted to cross‑react with all three species; however, always confirm specificity in your own model system using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated supplier for the IVD industry, CamelBio offers a comprehensive portfolio of validated raw materials including antibodies, antigens, and ancillary reagents. This POLD1 monoclonal antibody is suited for research into DNA replication fidelity, repair mechanisms, and cancer biology, and we can further assist with matched antibody pairs, bulk quantities, and sourcing for rare targets. Let CamelBio be your trusted partner from concept to clinic.