Anti-POFUT2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2G5

Core Product Specifications and Parameters

Biological Background: POFUT2 Function and Localization

• Protein Identity: GDP-fucose protein O-fucosyltransferase 2 (Peptide-O-fucosyltransferase 2), encoded by the POFUT2 gene, is a key enzyme in protein O-linked fucosylation.
• Core Function: Mediates O-fucosylation of thrombospondin type-1 repeats (TSRs) in the endoplasmic reticulum, catalyzing fucose attachment to serine/threonine within the consensus sequence C1-X-X-S/T-C2. Related references: PMID:22588082
• Substrate Proteins: Targets TSR-containing proteins including ADAMTS, thrombospondin, and spondin families, and is essential for proper secretion of ADAMTSL1 and ADAMTS13. Related references: PMID:17395588, PMID:17395589
• Subcellular Localization: Primarily located in the endoplasmic reticulum and Golgi apparatus.
• Isoform Diversity: Expressed as multiple isoforms via alternative splicing, with distinct tissue distributions.
• Tissue Specificity: Widely expressed; isoform A in fetal liver and peripheral blood lymphocytes, isoform B in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, and fetal brain, liver, kidney; isoform C in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, liver, and heart.
• Post-Translational Modifications & Keywords: Contains disulfide bonds, glycoprotein modifications, and a signal sequence; belongs to the glycosyltransferase family.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 240–370 of human POFUT2, suggesting reactivity toward the C-terminal region. Validate antibody specificity in your experimental system using appropriate controls.
• For Western blot (WB), a band at approximately 50 kDa is expected; consider optimizing blocking and antibody dilution based on your sample lysate.
• This antibody is reported to cross-react with mouse and rat, enabling multi-species studies. Confirm cross-reactivity in your model if not previously tested.
• Suitable for immunofluorescence/immunocytochemistry (IF/ICC) and ELISA; consider antigen retrieval and fixation conditions that preserve the target’s conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, connecting diagnostic developers with high-quality reagents. This anti-POFUT2 polyclonal antibody supports research into O-fucosylation, extracellular matrix biology, and related diseases. Beyond off-the-shelf products, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for even the rarest targets—streamlining your path from concept to clinic.