Anti-POF1B Rabbit Polyclonal Antibody for WB, ELISA - Q8WVV4

Core Product Specifications and Parameters

Biological Background: POF1B Function and Localization

• Protein POF1B (Premature ovarian failure protein 1B), encoded by the POF1B gene, is an actin-binding protein essential for the organization of epithelial monolayers.
• It primarily localizes to tight junctions at cell–cell contacts, where it likely links the actin cytoskeleton to junctional complexes to maintain epithelial integrity.
• The protein contains a coiled-coil domain and undergoes alternative splicing, producing multiple isoforms with potential functional diversity.
• POF1B may play a role in ovarian development; mutations in the gene are associated with premature ovarian failure (POF), a disorder of female infertility.
• Its molecular weight is approximately 68 kDa (calculated 68,065 Da), consistent with the observed band in Western blot.
• Keywords including ‘actin-binding’, ‘tight junction’, and ‘disease variant’ underscore its structural role in epithelia and disease relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 330–560 of human POF1B, which is a region likely exposed in the native protein; this partial sequence may help reduce cross-reactivity to other family members.
• For Western blot, consider using whole-cell lysates from human epithelial cell lines (e.g., HeLa, Caco-2) or rat tissue where POF1B is expressed. A band near 68 kDa should be detected; validate specificity using siRNA knockdown or peptide competition.
• In ELISA applications, direct coating of the recombinant immunogen or a peptide from the immunogenic region may serve as a positive control. Titrate the antibody to determine optimal working concentrations for your assay.
• Although cross-reactivity is reported for human and rat, always verify performance in your specific sample matrix and consider potential species-specific variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibodies, bulk ancillary reagents, and customized sourcing for rare targets like POF1B. For researchers focused on reproductive biology or epithelial junction dynamics, we provide reliable polyclonal antibodies and can support large-volume orders, helping you move from concept to clinic with confidence.