Anti-PODXL Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O00592

Core Product Specifications and Parameters

Biological Background: PODXL Function and Localization

• Podocalyxin (PODXL), also known as GCTM-2 antigen, Gp200, or podocalyxin-like protein 1 (PCLP-1), is a single-pass type I transmembrane glycoprotein belonging to the CD34 family. It is primarily expressed on glomerular podocytes and has roles in cell adhesion and morphology.
• Functions as both an anti-adhesive and pro-adhesive molecule: maintains an open filtration pathway between podocyte foot processes via charge repulsion, while in other contexts, it enhances integrin-dependent cell adhesion, migration, and cell-cell contacts.
• Induces the formation of apical actin-dependent microvilli and participates in establishing epithelial polarity and apical lumen formation during renal tubulogenesis.
• Contributes to cancer progression by promoting cell migration and invasion through interaction with the actin-binding protein EZR, leading to activation of MAPK and PI3K/AKT signaling pathways.
• Localizes to the apical cell membrane and is enriched in membrane projections such as microvilli, lamellipodia, filopodia, and ruffles, as well as membrane rafts.
• The protein undergoes post-translational modifications including phosphorylation and glycosylation, which may regulate its function. Alternative splicing generates multiple isoforms.
• Tissue specificity: predominantly expressed in glomerular epithelium (podocytes) of the kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular region (amino acids 300-479) of human PODXL; consider validating the antibody’s performance in your specific sample type using appropriate positive and negative controls.
• For Western blot (WB), the predicted molecular weight is approximately 59 kDa; optimize loading amounts and detection conditions to account for potential glycosylation-induced mobility shifts.
• In immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval and tissue fixation protocols should be optimized, as epitope accessibility can vary.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with another PODXL antibody for sandwich ELISA could be considered.
• Cross-reactivity with human PODXL is confirmed; reactivity with other species or isoforms should be determined experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This recombinant rabbit monoclonal antibody targeting PODXL is well-suited for investigations in podocyte biology, cell adhesion, and oncology research. As your sourcing partner, CamelBio offers validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw-material procurement for rare targets.