Anti-Podoplanin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q86YL7

Core Product Specifications and Parameters

Biological Background: Podoplanin Function and Localization

• Podoplanin (Aggrus, Gp36, PA2.26 antigen) is a small transmembrane glycoprotein of approximately 17 kDa (calculated molecular weight 16.7 kDa), with extensive O-glycosylation and sialic acid modifications.
• It mediates cell migration and adhesion through interactions with partners such as CLEC1B, CD9, MSN, EZR, and CD44; during development, it is critical for blood/lymphatic vessel separation by triggering platelet activation via CLEC1B binding. Related references: PMID:14522983 PMID:15231832 PMID:17222411
• Interaction with CD9 attenuates podoplanin-induced platelet aggregation. Related references: PMID:18541721
• Podoplanin promotes epithelial-mesenchymal transition (EMT) through MSN or EZR, leading to ERM phosphorylation, RHOA activation, and increased cell migration and invasiveness. Related references: PMID:17046996 PMID:21376833
• Interaction with CD44 enhances directional cell migration in epithelial and tumor cells. Related references: PMID:20962267
• Podoplanin controls invadopodia stability and maturation, facilitating extracellular matrix degradation in tumor cells via the RHOC-ROCK-LIMK-CFL1 pathway. Related references: PMID:25486435
• It localizes to the plasma membrane, particularly at membrane protrusions such as lamellipodia, filopodia, microvilli, ruffles, and invadopodia, and is also found in membrane rafts and cytosol.
• Highly expressed in placenta, lung, skeletal muscle, and brain; weakly in kidney and liver; up-regulated in colorectal tumors and a subset of oral squamous cell carcinomas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 99–199 of human Podoplanin, which includes a portion of the extracellular domain. This polyclonal antibody may recognize multiple epitopes within this region.
• For Western blot (WB), the predicted molecular weight is approximately 17 kDa, but extensive O-glycosylation can result in higher apparent molecular weights (40–45 kDa). Validate band specificity in the relevant sample system.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) and tissue-specific fixation conditions should be optimized to ensure specific staining.
• The antibody is listed as cross-reactive with mouse and rat Podoplanin; however, verification in the intended model system is recommended.
• For ELISA applications, the antibody can be used for antigen detection, but optimal coating and detection conditions should be determined experimentally.

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CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined one-stop channel for IVD raw-material sourcing, including optimized antibody reagents like this anti-Podoplanin polyclonal antibody. Beyond individual antibodies, CamelBio supplies validated antibody pairs, bulk ancillary reagents, and supports rare-target raw-material procurement across oncology, lymphatic biology, and cell adhesion research areas.