Anti-Podoplanin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q62011

Core Product Specifications and Parameters

Biological Background: Podoplanin Function and Localization

• Podoplanin (PDPN), also known as Gp38, OTS-8, E11, and T1alpha, is a type I transmembrane sialoglycoprotein of approximately 18 kDa expressed as an alternative PA2.26 antigen.
• It mediates cell migration and adhesion through diverse partners: binding CLEC1B triggers platelet activation/aggregation, crucial for blood-lymphatic vessel separation during development (PubMed:14522983, PubMed:15231832, PubMed:17616532). Related references: PMID:14522983 PMID:15231832 PMID:17616532
• Conversely, interaction with CD9 attenuates platelet aggregation and metastasis; interactions with MSN or EZR promote epithelial-mesenchymal transition (EMT) via ERZ phosphorylation and RHOA activation, increasing invasiveness.
• In lymph nodes, PDPN controls fibroblastic reticular cell (FRC) adhesion and contraction by maintaining activated ERM proteins and MYL9; CLEC1B engagement promotes relaxation (PubMed:25347465). Related references: PMID:25347465
• In keratinocytes, ectopic expression induces elongated morphology, actin reorganization, increased motility, and decreased adhesion (PubMed:10574709). Related references: PMID:10574709
• Subcellularly, it localizes to plasma membrane domains including lamellipodia, filopodia, microvilli, ruffles, membrane rafts, apical and basolateral membranes, and invadopodia, consistent with its role in cell migration and matrix degradation.
• Tissue distribution is highest in lung and brain, with lower levels in kidney, stomach, liver, spleen, esophagus; expressed in lymphatic endothelia, epithelial cells of choroid plexus, glomerulus, alveoli, and stromal cells of lymphoid tissues; up-regulated in colon carcinomas.
• It is required for normal lung cell proliferation and alveolus formation at birth (PubMed:12654292). Related references: PMID:12654292
• Additional keywords: cell junction, developmental protein, glycoprotein, sialic acid; it does not function as a water channel or folate transporter (PubMed:12032185). Related references: PMID:12032185

Experimental Guidance and Technical Tips

• For Western blotting, target the expected band at approximately 18 kDa, though glycosylation may cause a higher apparent molecular weight; include appropriate positive controls (e.g., lung or lymphatic tissue lysates).
• In immunohistochemistry and immunofluorescence, consider using tissues with known high Podoplanin expression (e.g., lung, lymphatic vessels) or relevant cell lines; antigen retrieval may be necessary for optimal staining.
• As a rabbit monoclonal antibody, consistent lot-to-lot performance is expected; validate in your specific experimental system to confirm suitability for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined supply channel for high-quality IVD raw materials, including validated antibody pairs and bulk ancillary reagents. This Podoplanin rabbit monoclonal antibody supports cancer and developmental biology applications from concept to clinical assay development, backed by our integrated procurement and technical support. For custom or rare-target sourcing, our platform bridges the gap between discovery and diagnostic deployment.