Anti-POC1A Polyclonal Antibody for WB, ELISA - Q8NBT0

Core Product Specifications and Parameters

Biological Background: POC1A Function and Localization

• POC1A, also known as Pix2 or WDR51A, is a WD repeat-containing protein that localizes to centrosomes and basal bodies, playing a critical role in centriole biogenesis.
• It is required for early centriole duplication and later steps of centriole length control, ensuring proper centriole integrity and mitotic spindle formation.
• Works in concert with its homolog POC1B to maintain centriole structure and support ciliogenesis.
• Subcellularly, POC1A is found at the centrosome, centriole, cilium basal body, and spindle pole during mitosis.
• Mutations in POC1A are associated with ciliopathies, including clinical features such as dwarfism and hypotrichosis.
• The protein undergoes alternative splicing and contains coiled-coil domains alongside its WD repeat motifs.
• The human POC1A gene encodes a protein with a predicted molecular weight of ~45 kDa, consistent with the immunogen design for this antibody.
• Given its cytoskeletal and cell projection roles, POC1A is a key target for studying centrosome biology and related disorders.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates from human, mouse, or rat cell lines; the predicted band is around 45 kDa. Validate in the relevant sample system.
• For ELISA, the recombinant immunogen (amino acids 138-407) can serve as a positive control to assess antibody reactivity.
• As a polyclonal antibody, it may recognize multiple epitopes, offering robust detection but requiring careful optimization for specific applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-POC1A reagent. We support centriole and ciliogenesis research by supplying bulk ancillary reagents and sourcing rare-target raw materials, all backed by technical and consulting services from concept to clinic.