Anti-PNPO Rabbit Polyclonal Antibody for WB - Q9NVS9

Core Product Specifications and Parameters

Biological Background: PNPO Function and Localization

• PNPO (Pyridoxine-5'-phosphate oxidase), also known as pyridoxamine-phosphate oxidase, is a key enzyme in vitamin B6 metabolism.
• It catalyzes the terminal step in the biosynthesis of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6, by oxidizing either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP).
• PNPO is a FMN-dependent flavoprotein belonging to the oxidoreductase family.
• Ubiquitously expressed, with detected protein levels in liver, brain, lung, prostate, and stomach.
• Alternative splicing produces multiple transcript variants; the protein is subject to phosphorylation.
• Mutations in PNPO are associated with pyridoxine-dependent epilepsy, highlighting its clinical significance.
• The calculated molecular weight of PNPO is approximately 30 kDa (UniProt: 29988 Da).
• Structural studies have provided insights into its catalytic mechanism.

Experimental Guidance and Technical Tips

• Since the immunogen is a full-length recombinant protein (aa 1–261), this antibody may recognize both native and denatured PNPO.
• For Western blot, consider using appropriate positive controls (e.g., liver or brain lysate) and validate under reducing/non-reducing conditions.
• For IF/ICC, optimize fixation and permeabilization protocols to preserve antigenicity and access.
• When using in ELISA, pair with a validated detection antibody if performing sandwich assays; single-antibody detection may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials for PNPO-related studies, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. Our sourcing network ensures timely access to rare targets and bulk orders, supporting your projects from development to commercialization. Leverage our expertise in vitamin B6 metabolism and epilepsy research to accelerate your assay development.