Anti-PNLIPRP2 Polyclonal Antibody for WB, IF-P, ELISA - P54317

Core Product Specifications and Parameters

Biological Background: PNLIPRP2 (PLRP2) Function and Localization

• PNLIPRP2, also designated pancreatic lipase-related protein 2 (PLRP2), is alternatively known as cytotoxic T lymphocyte lipase, galactolipase, and triacylglycerol lipase.
• Functions primarily as a lipase that hydrolyzes triglycerides and galactosylglycerides, with activity against short-, medium-, and long-chain fatty acyls without positional specificity. It can completely deacylate triacylglycerols and exhibits low phospholipase activity. Related references: PMID:15287741, PMID:17401110, PMID:18702514, PMID:21865348
• In neonates, plays a major role in pancreatic digestion of dietary fats, particularly milk fat globules enriched in long-chain triglycerides. Related references: PMID:19824014, PMID:21652702, PMID:23732775
• In adults, likely functions mainly as a galactolipase and monoacylglycerol lipase, hydrolyzing monogalactosyldiglycerols (MGDG) and digalactosyldiacylglycerols (DGDG) from plant-based diets, releasing long-chain polyunsaturated fatty acids. Related references: PMID:15287741, PMID:17401110, PMID:18702514, PMID:20083229
• May act together with gastric lipase (LIPF) to hydrolyze partially digested triglycerides. Related references: PMID:23732775
• In cytotoxic T cells, contributes to perforin-dependent cell lysis (by similarity). In neurons, required for the localization of the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) to neurite tips, promoting surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of transport vesicles with the plasma membrane (by similarity).
• Found as a secreted protein, associated with zymogen granule membranes, and localized to cell projections including neuron projections.
• Highly expressed in the pancreas; contains structural features such as calcium-binding sites, disulfide bonds, and glycosylation motifs.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 301–470 of human PNLIPRP2, covering a region within the mature protein. This likely generates antibodies recognizing multiple epitopes, suitable for detecting endogenous PNLIPRP2.
• For Western blotting (WB), expect a band near 52 kDa in reduced samples; validate detection in your specific tissue or cell lysate, and titrate antibody concentrations.
• In immunofluorescence (IF-P), PNLIPRP2 localizes to secretory vesicles and neuronal projections. Consider optimizing fixation and permeabilization to detect membrane-associated pools.
• For ELISA applications, pair with a suitable capture/detection antibody. Validate with recombinant PNLIPRP2 or cell supernatants to assess sensitivity.
• Cross-reactivity with mouse and rat enables translational studies, but verify performance in your model system as epitope conservation varies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive sourcing platform for IVD raw materials, technical support, and consulting—from concept to clinic. For studies on lipid metabolism, pancreatic function, and related research areas, we supply validated antibody pairs, polyclonal antibodies such as this anti-PNLIPRP2 antibody, bulk ancillary reagents, and custom sourcing of rare targets. Contact us to streamline your assay development with reliable reagents and expert guidance.