Anti-PNLIPRP1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P54315

Core Product Specifications and Parameters

Biological Background: PNLIPRP1 Function and Localization

• PNLIPRP1 (also known as PLRP1) is an inactive pancreatic lipase-related protein 1, belonging to the AB hydrolase superfamily.
• Unlike active lipases, PNLIPRP1 exhibits very low or undetectable catalytic activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids, or cholesterol esters (By similarity).
• In vitro, PNLIPRP1 is unable to hydrolyze milk fat, indicating its lack of lipolytic function under physiological conditions. Related references: PMID:19824014
• The PNLIPRP1 gene is subject to alternative splicing, potentially generating multiple isoforms (UniProt keyword).
• PNLIPRP1 is a secreted protein, consistent with its role in the pancreatic exocrine function.
• Tissue expression is predominantly in the pancreas, where it is produced alongside other pancreatic lipases.
• The protein structure includes features such as calcium-binding sites, disulfide bonds, and a signal peptide, characteristic of secreted lipases (Keywords: Calcium, Disulfide bond, Signal).
• While its biological role remains unclear, PNLIPRP1 may serve regulatory or structural functions in lipid metabolism.

Experimental Guidance and Technical Tips

• When performing Western blot, a band at approximately 52 kDa corresponding to PNLIPRP1 may be observed. Validate with appropriate positive controls such as pancreatic lysates.
• For immunohistochemistry, pancreas tissue sections are recommended due to the protein's tissue-specific expression.
• The immunogen covers the C-terminal region (aa 298–467); therefore, the antibody may detect full-length and possibly cleaved forms.
• Antibody cross-reactivity with mouse and rat has been reported; consider sequence alignment for other species to anticipate potential cross-detection.
• Standard blocking and detection protocols for rabbit polyclonal antibodies are applicable; optimization of antibody dilution and incubation conditions is advised for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs in pancreatic enzyme and metabolic disease research. We supply rigorously validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic. Whether you need high-quality anti-PNLIPRP1 antibodies or customized solutions for lipid metabolism targets, CamelBio bridges the gap between discovery and diagnostic application.