Anti-PNLIP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16233

Core Product Specifications and Parameters

Biological Background: Pancreatic Triacylglycerol Lipase (PNLIP) Function and Localization

• PNLIP encodes pancreatic triacylglycerol lipase, a member of the lipase family also known as PL, PTL, and PNLIPD.
• The enzyme plays a central role in dietary fat digestion by preferentially hydrolyzing the ester bonds at the sn-1 and sn-3 positions of triglycerides, yielding 2-monoacylglycerol and free fatty acids; it exhibits greater activity against emulsified lipid substrates.
• PNLIP is a secreted protein, released into the duodenum to facilitate lipid breakdown in concert with bile salts and colipase.
• The protein contains structural features including disulfide bonds, glycosylation sites, and a calcium-binding domain, which are important for its stability and enzymatic function.
• UniProt keywords associated with PNLIP include 'Disease variant', highlighting potential links to inherited metabolic or pancreatic disorders.

Experimental Guidance and Technical Tips

• The immunogen encompasses a large C-terminal fragment (aa 166–465) of human PNLIP, likely targeting conserved structural epitopes; cross-reactivity with mouse is reported.
• For Western blot, expect a specific band near 51 kDa in pancreatic tissue or cell lysates; include appropriate positive and negative controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and test on human or mouse pancreas sections to assess staining pattern.
• For indirect immunofluorescence (IF/ICC), confirm expression localization in pancreatic acinar cells or transfected cell lines.
• As a polyclonal antibody, background bands may be observed; validating in a tissue with known PNLIP expression (e.g., pancreas) is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material sourcing, offering a comprehensive range of validated antibodies, recombinant proteins, and ancillary reagents. For researchers focused on lipid metabolism and digestive enzyme studies, we supply premium tools like this anti-PNLIP rabbit polyclonal antibody, alongside customized bulk orders and rare-target material procurement to accelerate your diagnostic and assay development.