Anti-PNLIP Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P16233

Core Product Specifications and Parameters

Biological Background: PNLIP Function and Localization

• PNLIP (pancreatic triacylglycerol lipase) is a secreted enzyme essential for dietary fat digestion, predominantly produced in pancreatic acinar cells.
• It preferentially hydrolyzes ester bonds at the sn-1 and sn-3 positions of long-chain triglycerides, generating 2-monoacylglycerol and free fatty acids.
• The enzyme exhibits markedly higher catalytic activity on insoluble emulsified lipid substrates (e.g., dietary fats) compared to soluble substrates.
• Subcellular localization: Secreted into the duodenum upon hormonal stimulation, consistent with its role in nutrient breakdown.
• Structurally, it contains disulfide bonds, calcium-binding sites, and glycosylation modifications (as indicated by keywords such as Glycoprotein and Metal-binding), contributing to its stability and activity.
• Keywords: 3D-structure, Calcium, Disease variant (linked to lipid metabolism disorders), Disulfide bond, Glycoprotein, Hydrolase, Lipid degradation, Lipid metabolism, Metal-binding, Proteomics identification, Reference proteome, Secreted, Signal.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 51–52 kDa is expected under reducing conditions; glycosylation may cause minor size shifts. Use pancreas or intestinal tissue lysates as positive controls.
• The antibody is amenable to immunofluorescence on paraffin-embedded tissues (IF-P); consider antigen retrieval optimization for best results.
• ELISA performance may vary by plate coating and detection format; validate with recombinant PNLIP or known positive samples.
• The immunogen (aa 166-465) corresponds to the mature enzyme; thus, the antibody likely recognizes the active lipase form. Cross-reactivity with mouse and rat homologs has been confirmed, enabling multi-species studies.
• For functional assays, note that PNLIP requires colipase and bile salts for optimal activity; using complex biological samples may require additional controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic manufacturers streamlined access to a comprehensive portfolio of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For PNLIP and lipid metabolism targets, we supply optimized polyclonal antibodies and can assist with custom reagent sourcing. Whatever your project's stage—from concept to clinic—rely on CamelBio as a single, efficient procurement partner.