Anti-PNKP Rabbit Polyclonal Antibody for WB - Q96T60

Core Product Specifications and Parameters

Biological Background: PNKP Function and Localization

• PNKP is a bifunctional enzyme that acts as both a DNA 3'-phosphatase and a 5'-kinase, essential for the repair of DNA strand breaks. Related references: PMID:10446192, PMID:10446193
• It functions in two major DNA repair pathways: non-homologous end joining (NHEJ) and base excision repair (BER), processing damaged DNA termini for ligation. Related references: PMID:10446192, PMID:10446193, PMID:15385968
• PNKP is localized to the nucleus and associates with chromosomes, consistent with its role in DNA repair.
• The protein is expressed in many tissues, with highest expression in spleen and testis, and lowest in small intestine; it is also found at higher levels in pancreas, heart, and kidney. Related references: PMID:10446192, PMID:10446193
• PNKP belongs to the DNA repair enzyme family and is also known as DNA 5'-kinase/3'-phosphatase or polynucleotide kinase-3'-phosphatase.
• It contains key functional domains for ATP-binding and catalytic activity, and undergoes regulatory post-translational modifications including acetylation and phosphorylation.
• Mutations in PNKP are associated with neurological disorders such as ataxia-oculomotor apraxia 4 (AOA4), microcephaly with seizures (MCSZ), and Charcot-Marie-Tooth disease type 2B2 (CMT2B2), highlighting its critical role in neuronal DNA repair.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 60-320 of human PNKP (NP_009185.2), covering the core catalytic region; this sequence is highly conserved among human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot, a band around 57 kDa is expected. Use an appropriate gel percentage and membrane transfer protocol to resolve this protein.
• PNKP is a nuclear protein; in immunofluorescence (IF/ICC) or immunohistochemistry (IHC-P), expect nuclear staining. Include appropriate controls and antigen retrieval steps for best results.
• Confirm expression levels in your sample system, as PNKP is expressed broadly but with tissue-specific variations (high in spleen/testis, low in intestine).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop platform for IVD raw materials, including validated antibodies like this anti-PNKP polyclonal antibody for DNA repair research and assay development. Our portfolio encompasses monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing solutions for rare targets to accelerate your project from concept to clinic.