Anti-PNKP Rabbit Monoclonal Antibody for WB, ELISA - Q96T60

Core Product Specifications and Parameters

Biological Background: PNKP Function and Localization

• PNKP (bifunctional polynucleotide phosphatase/kinase) plays a crucial role in DNA damage repair by participating in non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Its dual 5'-kinase and 3'-phosphatase activities ensure DNA termini are compatible with ligation. Related references: PMID:10446192, PMID:10446193, PMID:15385968
• Also known as DNA 5'-kinase/3'-phosphatase or polynucleotide kinase-3'-phosphatase.
• The protein localizes to the nucleus and chromosomes (UniProt: SUBCELLULAR LOCATION).
• Expression is tissue-specific, with highest levels in spleen and testis, and lower levels in small intestine, brain, lung, and liver. Related references: PMID:10446192, PMID:10446193
• The protein has a calculated molecular weight of 57,076 Da, consistent with immunoblotting at ~57 kDa.
• Clinical mutations are associated with diseases such as microcephaly, seizures (MCSZ), and ataxia-oculomotor apraxia type 4 (AOA4), underscoring its neurological importance.
• Post-translational modifications include acetylation and phosphorylation, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (amino acids 400–500); verify that the target epitope is present in your experimental system, especially if using truncated constructs.
• For Western blotting, expect a band at approximately 57 kDa. Use appropriate molecular weight markers and validate with positive controls from human, mouse, or rat cell lines.
• For ELISA applications, consider testing the antibody as both capture and detection reagent, and optimize coating and detection antibody concentrations.
• While cross-reactivity is reported for mouse and rat, individual sample validation is recommended to confirm reactivity and signal specificity.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency; however, always perform initial validation for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive one-stop sourcing partner for IVD raw materials, dedicated to supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This Anti-PNKP antibody, targeting a key DNA repair protein, exemplifies our offering of validated monoclonal antibodies, alongside recombinant proteins, bulk reagents, and customized rare-target sourcing. Partner with CamelBio to streamline your assay development and secure high-quality critical raw materials.