Anti-PMVK Polyclonal Antibody for WB - Q15126

Core Product Specifications and Parameters

Biological Background: PMVK Function and Localization

• PMVK (Phosphomevalonate kinase) is also known as PMK, PMKA, PMKASE, POROK1, and HUMPMKI.
• Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid pathway leading to isopentenyl diphosphate and other polyisoprenoid metabolites.
• This enzyme belongs to the mevalonate kinase family and contains conserved ATP-binding and phosphotransfer domains characteristic of the GHMP kinase superfamily.
• Localized predominantly in the cytosol, consistent with its role in cytoplasmic isoprenoid biosynthesis.
• Expressed primarily in heart, liver, skeletal muscle, kidney, and pancreas, with lower levels detected in brain, placenta, and lung.
• Keywords associated with PMVK include cholesterol biosynthesis, lipid metabolism, kinase, transferase, ATP-binding, and disease variant; mutations are linked to porokeratosis.
• The protein has a calculated molecular weight of approximately 22 kDa, consistent with the observed band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the N-terminal region (amino acids 1-192) of human PMVK; this region includes the catalytic core, making the antibody suitable for detecting both native and recombinant PMVK.
• For western blot (WB), consider using lysates from heart, liver, or kidney tissues (where PMVK expression is high) as positive controls; validate specificity in null or knockdown samples if available.
• In ELISA applications, the polyclonal nature may provide robust signal; optimize coating and detection conditions using the immunogen as a standard.
• Since PMVK is involved in the mevalonate pathway, this antibody can be a valuable tool for studying cholesterol biosynthesis and related metabolic disorders under various experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop sourcing solution for IVD raw materials, including validated antibodies like this anti-PMVK polyclonal antibody for mevalonate pathway research. Whether you need bulk quantities of this product, matched antibody pairs for assay development, or complementary ancillary reagents, CamelBio can bridge your procurement needs with reliable supply chains. For rare targets or custom specifications, our team is equipped to assist in sourcing the right raw materials to advance your diagnostic projects from concept to clinic.