Anti-PMS2 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P54278

Core Product Specifications and Parameters

Biological Background: PMS2 Function and Localization

• PMS2 (PMS1 protein homolog 2) is a core component of the post-replicative DNA mismatch repair (MMR) system, where it heterodimerizes with MLH1 to form the MutL alpha complex. Related references: PMID:30653781 PMID:35189042
• Recruitment of MutL alpha to the mismatch is mediated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3), and assembly of the ternary complex with RFC and PCNA activates the latent endonuclease activity of PMS2, introducing single-strand breaks near the mismatch.
• The strand discrimination mechanism relies on DNA methylation: the newly synthesized, unmethylated strand is preferentially nicked, ensuring correction of replication errors.
• As an endonuclease, PMS2 generates entry points for exonuclease EXO1, which excises the error-containing strand, and it also physically interacts with DNA polymerase III clamp loader subunits, suggesting a role in recruiting the replicative polymerase for repair synthesis.
• Beyond MMR, PMS2 is implicated in DNA damage signaling, triggering cell cycle arrest or apoptosis in response to extensive DNA lesions, thereby acting as a tumor suppressor.
• PMS2 possesses intrinsic ATPase activity; however, ATP hydrolysis may not be essential for proficient mismatch repair under certain conditions. Related references: PMID:35189042
• The protein localizes exclusively to the nucleus and is phosphorylated at multiple sites. Mutations in PMS2 are associated with hereditary nonpolyposis colorectal cancer (Lynch syndrome) and other malignancies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 390–670 in the C-terminal region of human PMS2, which contains the endonuclease active site; consider validating reactivity in samples expressing full-length or truncated PMS2 variants.
• For Western blot (WB), expect a band at approximately 96 kDa; use a positive control such as HeLa or HEK293 cell lysates, and include nuclear extracts to enhance signal.
• In immunofluorescence (IF/ICC), PMS2 shows a nuclear staining pattern; co-staining with a nuclear marker is recommended to confirm localization.
• For immunoprecipitation (IP), the antibody may pull down the MutL alpha complex (MLH1-PMS2); validate co-precipitation in relevant cell lines.
• Cross-reactivity with mouse PMS2 is reported; for mouse studies, verify performance in the intended tissue or cell type.
• Optimize antibody dilutions for each application and sample system, as reactivity can vary depending on expression levels and post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories worldwide. The PMS2 antibody supports studies in DNA mismatch repair and cancer research, and we can assist with rare target raw-material procurement and custom development for specialized applications. From concept to clinic, CamelBio streamlines your supply chain to accelerate assay development.