Anti-PMS2 Rabbit Polyclonal Antibody for WB, ELISA - P54278

Core Product Specifications and Parameters

Biological Background: PMS2 Function and Localization

• PMS2 (PMS1 protein homolog 2), also known as MLH4, is a fundamental component of the post-replicative DNA mismatch repair (MMR) system. It forms the MutL alpha heterodimer with MLH1, which is recruited to mismatched DNA by the MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) complexes. Related references: PMID:30653781, PMID:35189042
• Upon assembly of the MutL-MutS-heteroduplex ternary complex, PMS2 exerts its endonuclease activity to introduce single-strand breaks near the mismatch, creating entry points for the exonuclease EXO1 to excise the error-containing strand.
• PMS2 possesses ATPase activity, although ATP hydrolysis may not be essential for proficient mismatch repair under certain conditions.
• Beyond MMR, PMS2 is implicated in DNA damage signaling, a process that can trigger cell cycle arrest or apoptosis in response to severe DNA lesions.
• The protein localizes predominantly to the nucleus, consistent with its role in genome maintenance.
• Alternative splicing generates multiple PMS2 isoforms, and the protein is subject to phosphorylation, which may modulate its function.
• Germline mutations in PMS2 are associated with hereditary nonpolyposis colorectal cancer (HNPCC, Lynch syndrome), positioning PMS2 as a tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human PMS2. For Western blotting, a predominant band near 96 kDa is expected; minor bands may arise from isoforms or post-translational modifications. Validate detection in your specific sample system.
• Cross-reactivity with rat PMS2 has been demonstrated. When working with other species, assess homology and perform a pilot experiment to confirm reactivity.
• For ELISA applications, optimize coating antigen and antibody dilutions empirically. Include appropriate controls to ensure specificity.
• Given PMS2’s involvement in multiple complexes, consider the possibility of co-immunoprecipitation applications if the antibody recognizes native protein; however, such usage requires thorough validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-PMS2 rabbit polyclonal antibody is a valuable tool for investigating mismatch repair mechanisms and developing cancer diagnostics. In addition to validated antibodies, we offer optimized monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and customized sourcing for rare or challenging targets—all designed to streamline your assay development pipeline.