Anti-PMS2 Rabbit Monoclonal Antibody (KO Validated) for WB and ELISA - P54278

Core Product Specifications and Parameters

Biological Background: PMS2 Function and Localization

• PMS2 (PMS1 protein homolog 2), also termed MLH4, PMS-2, PMSL2, HNPCC4, LYNCH4, or MMRCS4, is a 96 kDa mismatch repair endonuclease essential for maintaining genomic stability.
• It is a key component of the post-replicative DNA mismatch repair (MMR) system, forming a heterodimer with MLH1 (MutL alpha). This complex is recruited to mismatch sites by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) upon recognition of base-base mismatches or insertion/deletion loops. Related references: PMID:30653781, PMID:35189042
• Upon recruitment, PMS2 endonuclease activity introduces single-strand breaks near the mismatch, creating entry points for EXO1 exonuclease to degrade the error-containing strand. DNA methylation likely directs cleavage to the newly synthesized strand, ensuring strand-specific repair.
• PMS2 also possesses ATPase activity; however, ATP hydrolysis may not be required for proficient mismatch repair as gross conformational changes are absent.
• Beyond mismatch repair, PMS2 is implicated in DNA damage signaling, promoting cell cycle arrest and apoptosis in response to severe DNA lesions.
• The protein localizes predominantly in the nucleus, consistent with its DNA repair functions, and undergoes alternative splicing, leading to multiple isoforms.
• Mutations in PMS2 are linked to hereditary nonpolyposis colorectal cancer (HNPCC, Lynch syndrome) and other mismatch repair cancer syndromes, classifying it as a tumor suppressor.

Experimental Guidance and Technical Tips

• For Western blotting, the target protein runs at approximately 96 kDa; consider using fresh lysates from human colorectal cell lines (e.g., HCT116, SW480) as positive controls and validate knockout/knockdown models to confirm band specificity.
• For ELISA applications, optimization of coating antigen and antibody concentration is recommended; test with recombinant human PMS2 protein to assess sensitivity and linearity.
• Since the antibody is produced against recombinant human PMS2 protein, cross-reactivity with other species is not expected; if attempting other species, perform sequence alignment and validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined one-stop supply of high-quality IVD raw materials, from validated monoclonal antibodies to bulk ancillary reagents. For DNA mismatch repair targets like PMS2, our portfolio includes optimized antibody pairs and ready-to-use reagents suitable for oncology and genetic testing assays. We also offer custom sourcing for rare targets, technical consultation, and rapid scaling from concept to clinical application.