Anti-PMS1 Rabbit Polyclonal Antibody for WB, ELISA - P54277

Core Product Specifications and Parameters

Biological Background: PMS1 Function and Localization

• PMS1 (PMS1 protein homolog 1), also known as DNA mismatch repair protein PMS1, is encoded by the PMS1 gene (Gene ID: 5378). Alternative splicing generates multiple isoforms, with the canonical form having a molecular weight of approximately 106 kDa (UniProt: 105.83 kDa).
• PMS1 is probably involved in the repair of DNA mismatches, a critical process for maintaining genomic stability by correcting base-base and insertion-deletion mispairs that arise during DNA replication.
• As a component of the mismatch repair system, PMS1 functions in concert with other MMR proteins and is associated with hereditary nonpolyposis colorectal cancer (HNPCC) when mutated.
• PMS1 localizes predominantly to the nucleus, where it accesses DNA to perform its repair functions.
• The protein contains DNA-binding domains, consistent with its role in recognizing and processing mismatched DNA segments.
• PMS1 is classified as a tumor suppressor, and its dysfunction is linked to DNA damage and tumorigenesis.

Experimental Guidance and Technical Tips

• The polyclonal antibody is raised against a recombinant immunogen corresponding to the C-terminal region (aa 405-619) of human PMS1. For Western blot, consider using nuclear lysates from human cell lines, as PMS1 is a nuclear protein with an expected band around 106 kDa.
• For ELISA, the antibody may recognize the immunogen region; consider coating plates with the recombinant protein or nuclear extracts from human samples.
• Cross-reactivity is reported for human; for other species, sequence alignment of the immunogen region is recommended to predict potential cross-reactivity.
• Validation in mismatch repair-proficient and -deficient cell models may provide additional specificity controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This PMS1 rabbit polyclonal antibody supports research in DNA repair and cancer diagnostics. Our supply capabilities include validated antibody pairs, bulk ancillary reagents, and sourcing of rare-target raw materials for your assay development needs.