Anti-PMPCB Rabbit Polyclonal Antibody for WB, ELISA - O75439

Core Product Specifications and Parameters

Biological Background: PMPCB Function and Localization

• Mitochondrial-processing peptidase subunit beta (PMPCB), also known as Beta-MPP or P-52, is the catalytic subunit of the essential mitochondrial processing protease (MPP).
• Catalytic subunit of MPP, which cleaves the mitochondrial targeting sequences from newly imported precursor proteins after their translocation into the mitochondrial matrix. Related references: PMID:29576218
• Preferentially cleaves after an arginine at position P2 in the presequence, a critical determinant for substrate recognition.
• Required for PINK1 turnover by coupling mitochondrial import and proteolytic cleavage, leading to subsequent PINK1 degradation and regulating mitophagy. Related references: PMID:22354088
• Localized to the mitochondrial matrix, consistent with its role in processing nuclear-encoded mitochondrial precursor proteins.
• Contains a zinc-binding metalloprotease domain and is classified as both a hydrolase and metalloprotease; its activity is metal-dependent.
• Linked to neurodegenerative processes through disease variant associations, highlighting its importance in neuronal mitochondrial health.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 54 kDa is expected; consider using mitochondrial-enriched lysates to enhance the signal.
• The antibody is validated to cross-react with Human, Mouse, and Rat species, making it suitable for comparative studies.
• For ELISA, the recombinant immunogen covering amino acids 110-380 can serve as a positive control to assess binding efficiency.
• Optimize antibody dilution and incubation conditions based on your specific sample type and experimental system; titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-PMPCB polyclonal antibody supports research into mitochondrial processing and neurodegenerative disease mechanisms. In addition to validated antibodies, CamelBio can supply bulk ancillary reagents and assist in sourcing rare-target raw materials to streamline your diagnostic development needs.