Anti-PMP70/ABCD3 Monoclonal Antibody for WB, IF/ICC, ELISA - P28288

Core Product Specifications and Parameters

Biological Background: PMP70/ABCD3 Function and Localization

• ABCD3/PMP70 is a peroxisomal membrane protein belonging to the ATP-binding cassette (ABC) transporter subfamily D, critical for the import of fatty acids and bile acids into peroxisomes.
• It catalyzes the ATP-dependent translocation of long-chain fatty acyl-CoAs, dicarboxylic acids, and branched-chain fatty acids across the peroxisomal membrane, a key step in beta-oxidation. Related references: PMID:11248239, PMID:24333844, PMID:25168382
• ABCD3 possesses intrinsic fatty acyl-CoA thioesterase activity, likely converting acyl-CoA substrates to free fatty acids before transport. Related references: PMID:29397936
• The protein plays a pivotal role in energy homeostasis by regulating the peroxisomal beta-oxidation pathway, thereby influencing lipid metabolism and degradation. Related references: PMID:24333844, PMID:25944712
• It localizes exclusively to the peroxisomal membrane, with its transporter domains oriented to facilitate vectorial movement of substrates from the cytosol into the organelle.
• ABCD3 is subject to post-translational modifications, including acetylation and phosphorylation, and is glycosylated, which may regulate its stability or function.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning residues 560–659, which may confer epitope specificity to the C-terminal region of ABCD3; consider using a blocking peptide to confirm specific binding.
• For Western blotting, a band at approximately 75 kDa is expected; validate detection in relevant cell lines or tissues expressing ABCD3, such as liver or peroxisome-rich samples.
• In IF/ICC, optimize permeabilization conditions to detect peroxisomal membrane localization; co-staining with peroxisomal markers may aid interpretation.
• The antibody is validated for human, mouse, and rat cross-reactivity; ensure appropriate species-specific controls when testing other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-ABCD3 clone. Whether you need bulk peroxisomal research reagents, customized antibody conjugation, or rare-target sourcing, our integrated supply chain accelerates your development from biomarker discovery to clinical application.