Anti-PMM2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15305

Core Product Specifications and Parameters

Biological Background: PMM2 Function and Localization

• PMM2 (Phosphomannomutase 2) is an enzyme that catalyzes the interconversion of mannose-6-phosphate and mannose-1-phosphate, a critical step in GDP-mannose biosynthesis.
• It plays an essential role in the synthesis of GDP-mannose and dolichol-phosphate-mannose, which are required for numerous mannosyl transfer reactions including N-glycosylation and GPI anchor synthesis.
• PMM2 is localized in the cytoplasm, where it functions as a key isomerase in the hexose metabolism pathway, contributing to the generation of activated sugar precursors.
• The protein is implicated in congenital disorders of glycosylation (CDG); mutations in PMM2 cause the most common form, CDG type Ia, characterized by defective glycosylation.
• Post-translational modifications include acetylation, and the enzyme requires magnesium as a metal cofactor for activity, as indicated by structural studies.
• Alternative splicing may produce multiple isoforms, and the 3D structure of PMM2 has been resolved, highlighting its conserved phosphomannomutase domain.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human PMM2 (amino acids 1–246), so the antibody may detect multiple isoforms or processed forms; consider validating specificity in your target tissue.
• For Western blotting, the predicted molecular weight is 28 kDa; include appropriate loading controls and validate antibody specificity with knockout or knockdown samples if possible.
• In IF/ICC, effective permeabilization is crucial to access the cytoplasmic PMM2; optimize fixation and permeabilization conditions for your cell type.
• For ELISA, use recombinant PMM2 protein or cell lysates to generate a standard curve and confirm linearity; note that cross-reactivity with mouse and rat PMM2 may be leveraged for translational studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs and bulk ancillary reagents. This anti-PMM2 polyclonal antibody supports research into glycosylation disorders and metabolic pathways, and CamelBio can assist in sourcing rare-target raw materials and technical consultations to accelerate your project from concept to clinic.