Anti-PML Polyclonal Antibody for WB, IHC-P, ELISA - P29590

Core Product Specifications and Parameters

Biological Background: PML Function and Localization

• PML (Promyelocytic leukemia protein), also designated TRIM19, MYL, and RNF71, is a member of the tripartite motif (TRIM) family and functions as an E3 SUMO-protein ligase.
  Related references:
  PMID:20972456 PMID:28250117

• PML serves as the scaffold of PML nuclear bodies (PML-NBs), subnuclear organelles that regulate key processes such as tumor suppression, transcriptional control, apoptosis, senescence, and DNA damage repair.

• It suppresses mRNA export by inhibiting the cap-binding protein EIF4E, reducing its affinity for the 5' 7-methylguanosine cap of target mRNAs.
  Related references:
  PMID:11500381 PMID:11575918 PMID:18391071

• Multiple isoforms generated by alternative splicing display specialized functions: PML-4 represses TBX2 during senescence and modulates p53 stability, while PML-6 specifically inhibits the tetrameric form of PKM.

• PML exhibits broad antiviral activity, restricting DNA and RNA viruses such as herpesviruses, influenza A, rabies, and poliovirus via capsid sequestration, polymerase trapping, or apoptosis induction.

• PML localizes to the nucleus, nucleoplasm, PML bodies, nucleolus, and also dynamically associates with cytoplasmic structures such as the endoplasmic reticulum and endosomes, enabling its multitasking roles.

• Post-translational modifications, including acetylation, phosphorylation, and SUMOylation, tightly regulate PML’s activity and its interactions within PML-NBs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 571–611 of human PML (isoform NP_150241.2); consult isoform alignment to anticipate potential cross-reactivity among splicing variants.
• For Western blot, a band near 98 kDa is expected, but additional bands from other isoforms or post‑translational modifications may appear; validate using known positive and negative controls.
• For immunohistochemistry (IHC-P), consider testing on tissues known to express PML, such as spleen, thymus, or tumor biopsies, and include appropriate controls.
• As a polyclonal antibody, lot‑to‑lot consistency is important; perform titration experiments to determine the optimal working dilution for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD manufacturers and laboratories, offering comprehensive raw-material sourcing, technical consultancy, and custom development services from concept to clinical application. With the PML polyclonal antibody, researchers gain a valuable tool for studying tumor suppression, viral defense, and apoptosis pathways, applicable in assay development for oncology and infectious disease diagnostics. Contact our team for bulk antibodies, matched antigen pairs, or tailored raw-material solutions to accelerate your diagnostic product pipeline.