Anti-PMF1 Monoclonal Antibody for WB, ELISA - Q6P1K2

Core Product Specifications and Parameters

Biological Background: PMF1 Function and Localization

• PMF1 (Polyamine-modulated factor 1) is a component of the MIS12 complex, which is essential for proper chromosome alignment, segregation, and kinetochore formation during mitosis.
• It may also function as a cotranscription partner of NFE2L2, playing a role in the regulation of polyamine-induced transcription of SSAT (spermidine/spermine N1-acetyltransferase).
• The protein is localized to the nucleus, specifically at the centromere and kinetochore of chromosomes, consistent with its mitotic function.
• PMF1 exhibits tissue-specific expression, with highest levels in heart and skeletal muscle, and significant expression in kidney and liver.
• The protein contains coiled-coil domains and undergoes alternative splicing, producing multiple isoforms.
• Structural studies have elucidated the assembly of PMF1 within the MIS12 kinetochore complex.
• Additional keywords from curated databases highlight roles in cell cycle, mitosis, and transcription regulation, underscoring its dual function in chromosome dynamics and gene expression.

Experimental Guidance and Technical Tips

• The antibody is generated against a recombinant protein corresponding to amino acids 1-205 of human PMF1, covering the entire predicted protein; it may detect endogenous PMF1 in human and rat samples by Western blot.
• For WB analysis, a band around 23kDa is expected; validate in cell lines or tissues with known PMF1 expression, such as HeLa (human) or heart/skeletal muscle lysates; a loading control is recommended.
• For ELISA, consider using the immunogen as a standard, and assess cross-reactivity with related MIS12 complex components if needed.
• Since PMF1 is a nuclear protein, appropriate subcellular fractionation may be considered for enriched detection.
• Optimize antibody dilution in the relevant experimental system, as the provided data does not include specific working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive raw-material sourcing solutions for IVD development, from concept to clinic. For research on chromosome segregation and polyamine metabolism, we offer validated monoclonal antibodies like anti-PMF1, along with polyclonal pairs and ancillary reagents to support your assay development. Our team specializes in bridging the gap between discovery and diagnostic manufacturing, ensuring reliable access to rare-target raw materials.