Anti-PMEPA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q969W9

Core Product Specifications and Parameters

Biological Background: PMEPA1 Function and Localization

• PMEPA1 gene encodes Protein TMEPAI (also known as STAG1, TMEPAI), which undergoes alternative splicing to produce multiple isoforms.
• Functions as a negative regulator of TGF-beta signaling by interacting with SMAD2 and SMAD3 and competing with ZFYVE9/SARA and SMAD4, thereby inhibiting signal transduction. Related references: PMID:20129061 PMID:24627487
• Enhances ubiquitination and proteasome-mediated degradation of the androgen receptor (AR) by recruiting NEDD4, providing a link to androgen signaling pathways. Related references: PMID:18703514
• Localizes primarily to the early endosome membrane and Golgi apparatus membrane, consistent with its role in receptor trafficking and signaling modulation.
• Exhibits highest expression in the prostate, with additional expression in the ovary, underscoring its relevance in reproductive tissues and hormone-related cancers.
• Classified as a signal transduction inhibitor and involved in the ubiquitin conjugation pathway, reflecting its dual regulatory roles in growth factor signaling and protein turnover.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 32 kDa (predicted molecular weight 31.6 kDa). Validate using positive control lysates from prostate or ovarian cell lines.
• For immunohistochemistry (IHC-P), consider using prostate or tumor tissue sections, where PMEPA1 is highly expressed. Optimize antigen retrieval and antibody dilution.
• For ELISA, this polyclonal antibody can serve as a capture or detection reagent when paired with appropriate standards; validate linearity and sensitivity in your assay buffer.
• The immunogen corresponds to amino acids 173–252, which is predicted to be part of the cytoplasmic domain. Ensure epitope preservation under your experimental conditions.
• As a polyclonal antibody, it may recognize multiple isoforms arising from alternative splicing; verify specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop source for IVD raw materials, streamlining procurement from discovery to clinical application. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies (such as this Anti-PMEPA1 pAb), and bulk ancillary reagents, with specialized expertise in rare and research-use targets for the cell signaling and cancer diagnostic space. Partner with us to accelerate your assay development with reliable, high-quality materials.