Anti-PMEPA1 Rabbit Polyclonal Antibody for WB and ELISA - Q969W9

Core Product Specifications and Parameters

Biological Background: PMEPA1 Function and Localization

• PMEPA1 is a transmembrane protein, also known as TMEPAI or STAG1, encoded by the PMEPA1 gene. Alternative splicing produces multiple isoforms.
• It negatively regulates TGF‑β signaling by binding to SMAD2 and SMAD3, competing with ZFYVE9/SARA and SMAD4, and inhibiting signal transduction. Related references: PMID:20129061, PMID:24627487
• PMEPA1 also down‑regulates the androgen receptor (AR) by enhancing its ubiquitination and proteasome‑mediated degradation, likely through NEDD4 recruitment. Related references: PMID:18703514
• The protein localizes primarily to early endosome and Golgi apparatus membranes.
• It is highly expressed in prostate tissue and also found in the ovary.
• PMEPA1 is classified under keywords including alternative splicing, signal transduction inhibitor, and transmembrane helix, reflecting its regulatory roles and structural features.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 173–252 of human PMEPA1; the antibody may recognize epitopes within this region.
• For WB, a band around 32 kDa is expected. Validate specificity using appropriate positive (e.g., prostate‑derived) and negative controls.
• In ELISA, consider optimizing coating antigen and detection conditions; cross‑reactivity with mouse and rat orthologs permits multispecies studies.
• Due to PMEPA1’s membrane localization, ensure lysis buffers are compatible with extracting transmembrane proteins.
• Given its role in TGF‑β and AR pathways, the antibody may be useful in cancer‑related signaling studies; confirm performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one‑stop partner for IVD raw materials, offering validated polyclonal antibodies like this anti‑PMEPA1 to support cancer and TGF‑β signaling research. We provide comprehensive sourcing solutions, including optimized antibody pairs, bulk reagents, and access to rare target raw materials, streamlining your journey from concept to clinic.