Anti-PMEL (gp100) Monoclonal Antibody for WB, IHC-P, ELISA - P40967

Core Product Specifications and Parameters

Biological Background: PMEL Function and Localization

• Melanocyte protein PMEL (also known as gp100, HMB-45, or SILV) is a premelanosome protein that plays a central role in melanosome morphogenesis and pigmentation.
• During melanosome maturation, processed amyloidogenic fragments of PMEL assemble into parallel fibrillar sheets, driving the elongation of stage I premelanosomes into striated ellipsoidal stage II melanosomes.
• In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform for concentrating eumelanin precursors, facilitating efficient melanin synthesis while potentially sequestering toxic reaction intermediates.
• PMEL is a well-characterized melanoma-specific antigen; immunodominant peptides derived from PMEL, such as G9-154 (KTWGQYWQV) and G9-209 (ITDQVPFSV), are presented by HLA-A molecules and stimulate anti-tumor cytotoxic T cell responses.
• Subcellularly, PMEL localizes to the endoplasmic reticulum membrane, Golgi apparatus, cis-Golgi network membrane, endosomes (multivesicular bodies), and melanosomes, and is also found in extracellular vesicles and secreted forms.
• The protein undergoes extensive post-translational modifications, including cleavage on pairs of basic residues, disulfide bond formation, glycosylation, and sialic acid addition.
• Normally expressed at low levels in quiescent adult melanocytes, PMEL is overexpressed by proliferating neonatal melanocytes and during tumor growth, particularly in melanomas and some dysplastic nevi.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 25-211) of human PMEL, which is predicted to be extracellular/luminal; consider this when designing detection strategies or pairing with other antibodies.
• For Western blotting, the full-length unprocessed PMEL migrates at approximately 70 kDa, but note that endogenous PMEL is proteolytically processed into multiple fragments; validate the expected band pattern in your lysates.
• In immunohistochemistry (paraffin), PMEL staining should be membranous/cytoplasmic in melanocytes and melanoma cells; include appropriate positive (melanoma tissue) and negative controls.
• For ELISA applications, use recombinant PMEL protein or synthetic peptides as coating antigens and carefully titrate the antibody to achieve optimal signal-to-noise ratio.
• Since PMEL expression can vary with melanocyte activation state, consider using known melanoma cell lines (e.g., SK-MEL-28) or primary melanocytes as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your diagnostic development by providing a one-stop platform for IVD raw materials. Whether you need validated monoclonal antibodies like this anti-PMEL clone, or are developing assays for melanoma markers, we offer optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to streamline your path from concept to clinic.