Anti-PMCH Rabbit Polyclonal Antibody for WB, ELISA - P20382

Core Product Specifications and Parameters

Biological Background: PMCH Function and Localization

• PMCH encodes the precursor protein pro-MCH, which is proteolytically processed to produce active neuropeptides including melanin-concentrating hormone (MCH), neuropeptide EI (NEI), and neuropeptide GE (NGE).
• MCH is a high-affinity ligand for G protein-coupled receptors MCHR1 and MCHR2, activating both G(i/o) and G(q/11) signaling pathways. Related references: PMID:15340116, PMID:38710677, PMID:39616153
• MCH acts as an orexigenic neuromodulator, promoting feeding and positive energy balance. Related references: PMID:15340116, PMID:38710677, PMID:39616153
• MCH promotes sleep and inhibits stimulated ACTH release, thereby down-regulating the HPA axis. Related references: PMID:15340116, PMID:38710677, PMID:39616153
• MCH functionally antagonizes alpha-MSH, counteracting its anorexigenic effects and stimulatory influence on the HPA axis. Related references: PMID:15340116, PMID:38710677, PMID:39616153
• NEI, another processed peptide from the same precursor, stimulates ACTH secretion and modulates stress responses, functionally opposing MCH's suppressive effects.
• PMCH is predominantly expressed in the lateral hypothalamus, with additional expression in other brain regions and peripheral tissues such as testis and duodenum.
• The pro-MCH precursor contains key structural features including a disulfide bond and amidation sites, essential for mature peptide bioactivity.

Experimental Guidance and Technical Tips

• For Western blot, the antibody may detect the ~19 kDa pro-MCH precursor; processed peptides (MCH ~2.4 kDa, NEI/GE) may require optimization of gel percentage and transfer conditions.
• Validate antibody performance in relevant human or mouse tissue lysates (e.g., hypothalamus, testis) due to reported cross-reactivity.
• For ELISA, consider using recombinant PMCH protein (e.g., immunogen) as a standard and titrate antibody concentration for optimal signal-to-noise ratio.
• As a polyclonal antibody, each lot may exhibit slight variations; it is advisable to include appropriate positive and negative controls in each experiment.
• The antibody is suitable for WB and ELISA; other applications have not been tested and may require additional optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-PMCH polyclonal antibody as part of a comprehensive portfolio of IVD raw materials for neuroendocrine and metabolic disease research. We supply validated antibody pairs, recombinant proteins, and ancillary reagents to support assay development. Discover how our integrated sourcing solutions can accelerate your diagnostic pipeline from concept to clinic.