Anti-PLZF Rabbit Monoclonal Antibody for WB - Q05516

Core Product Specifications and Parameters

Biological Background: PLZF Function and Localization

• PLZF (Promyelocytic leukemia zinc finger protein), also known as Zinc finger and BTB domain-containing protein 16 (ZBTB16) or Zinc finger protein 145 (ZNF145), is a transcriptional repressor encoded by the ZBTB16 gene.
• It functions as a transcriptional repressor, partly by recruiting histone deacetylases to target gene promoters (PubMed:10688654, PubMed:24359566). Related references: PMID:10688654 PMID:24359566
• PLZF is a substrate-recognition component of an E3 ubiquitin-protein ligase complex, mediating ubiquitination and proteasomal degradation of target proteins (PubMed:14528312). Related references: PMID:14528312
• The protein predominantly localizes to the nucleus, particularly in nuclear bodies, consistent with its role in transcriptional regulation.
• Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines, and peripheral blood mononuclear cells, with additional expression in ovary, kidney, and lung.
• PLZF contains zinc finger and BTB/POZ domains, classifying it as a metal-binding, DNA-binding transcription factor; it undergoes phosphorylation and is linked to the ubiquitin conjugation pathway.
• Alternative splicing generates multiple isoforms, and chromosomal rearrangements involving ZBTB16 are associated with acute promyelocytic leukemia.

Experimental Guidance and Technical Tips

• For Western blotting, PLZF has a predicted molecular weight of ~74 kDa. Use an appropriate gel percentage (e.g., 10%) and optimize transfer conditions for efficient detection.
• The immunogen (amino acids 199–300) resides in the protein’s central region. Check sequence conservation across species to anticipate potential cross-reactivity.
• This antibody is compatible with IP and IF-P; for nuclear antigens, consider proper fixation and permeabilization protocols to ensure nuclear access.
• Reported cross-reactivity with mouse and rat PLZF should be validated in the target sample system, as recognition may vary with endogenous expression levels.
• In ELISA, preliminary checkerboard titrations with a matched detection antibody are recommended to establish optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, offering diagnostic manufacturers and researchers a streamlined path from concept to clinic. For transcription factor targets like PLZF, we supply validated monoclonal antibodies, bulk ancillary reagents, and custom procurement of rare raw materials. Our extensive portfolio accelerates assay development with high-quality, well-characterized reagents for various research and clinical applications.