Anti-PLZF Polyclonal Antibody for WB, IHC-P, ELISA - Q05516

Core Product Specifications and Parameters

Biological Background: PLZF Function and Localization

• PLZF, encoded by the ZBTB16 gene, is a zinc finger and BTB domain-containing transcriptional repressor, also known as promyelocytic leukemia zinc finger protein (ZNF145).
• It mediates transcriptional repression through recruitment of histone deacetylases to target promoters, regulating myeloid maturation and other differentiation processes. Related references: PMID:10688654, PMID:24359566
• Functions as a substrate-recognition component of an E3 ubiquitin-protein ligase complex, directing ubiquitination and proteasomal degradation of target proteins. Related references: PMID:14528312
• It localizes predominantly in the nucleus and nuclear bodies.
• PLZF is expressed in hematopoietic tissues including bone marrow, early myeloid cell lines, and peripheral blood mononuclear cells, with lower expression in ovary, kidney, and lung.
• The protein features nine zinc-finger domains and a BTB/POZ domain, and is classified under metal-binding, DNA-binding, and alternative splicing categories, with involvement in chromosomal rearrangements linked to disease.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 199–300, located between the BTB domain and the zinc-finger repeats, potentially generating antibodies that recognize the native and denatured protein in various applications.
• For Western blot, consider a starting dilution of 1:500–1:2000 and validate using lysates from bone marrow or myeloid cell lines where PLZF is highly expressed.
• In immunohistochemistry (paraffin-embedded sections), perform heat-induced antigen retrieval and titrate the antibody to minimize background; include nuclear staining controls.
• For ELISA, use recombinant PLZF or peptide antigen as capture, and confirm species cross-reactivity if using mouse or rat samples.
• The polyclonal nature may yield a stronger signal but requires careful lot-to-lot consistency checks for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for one-stop sourcing of high-quality IVD raw materials, from concept to clinic. This anti-PLZF antibody supports research in transcription regulation, myeloid leukemia, and developmental biology, and CamelBio can supply validated antibody pairs, bulk reagents, and rare-target monoclonal/polyclonal antibodies to meet your diagnostic development needs. Contact our team to discuss custom antibody production, optimized ancillary reagents, and flexible supply solutions tailored to your project.