Anti-PLXNA3 Rabbit Polyclonal Antibody for WB, ELISA - P51805

Core Product Specifications and Parameters

Biological Background: PLXNA3 Function and Localization

• PLXNA3, also known as Plexin-A3, Plexin-4, or Semaphorin receptor SEX, is a single-pass transmembrane receptor belonging to the plexin family.
• It acts as a coreceptor for class 3 semaphorins SEMA3A and SEMA3F, forming signaling complexes with neuropilins to mediate cytoskeletal reorganization.
• PLXNA3 is essential for axon guidance and neuronal migration in the developing nervous system, regulating sympathetic neuron migration and dendritic spine morphology in pyramidal neurons.
• The protein may also be involved in semaphorin-mediated programmed cell death during neurodevelopment.
• PLXNA3 is localized at the cell membrane, with a large N-terminal extracellular region containing sema and PSI domains, a transmembrane helix, and a cytoplasmic tail required for downstream signaling.
• It undergoes glycosylation, disulfide bond formation, and phosphorylation, consistent with its role as a cell-surface receptor involved in signal transduction.

Experimental Guidance and Technical Tips

• Based on the immunogen sequence (amino acids 950–1150), the antibody is expected to recognize the cytoplasmic domain of human PLXNA3.
• For Western blot (WB), a band at approximately ~208 kDa is anticipated; due to the high molecular weight, consider using gradient gels and extended transfer times for optimal resolution and blotting.
• Validate the antibody in relevant human cell lines or tissues known to express PLXNA3 to confirm specificity.
• For ELISA, the antibody can be employed in both direct and capture formats; optimization of coating concentrations and detection systems is recommended.
• Cross-reactivity with human PLXNA3 is specified; for samples from other species, sequence homology should be examined, and experimental validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive provider of IVD raw materials, offering manufacturers and research laboratories access to validated antibodies, antigen pairs, and ancillary reagents. Our PLXNA3 polyclonal antibody supports neuroscience and cell signaling research applications. Whether you need bulk monoclonal antibodies, recombinant proteins, or rare-target sourcing, CamelBio bridges the gap from concept to clinic with reliable supply solutions.