Anti-PLVAP Polyclonal Antibody for WB, ELISA - Q9BX97

Core Product Specifications and Parameters

Biological Background: PLVAP Function and Localization

• PLVAP (plasmalemma vesicle-associated protein), also known as PV-1 (plasmalemma vesicle protein 1) or fenestrated endothelial-linked structure protein, is a single-pass type II transmembrane glycoprotein essential for endothelial cell-specific membrane structures.
• It is a key structural component of the diaphragms that bridge endothelial fenestrae, the stomata of caveolae, and transendothelial channels, thereby regulating microvascular permeability.
• PLVAP mediates the passage of water and solutes through fenestrae and controls the balance between transcellular and paracellular transport in various organs.
• The protein plays a specific role in embryonic development, likely due to its critical function in establishing proper vascular permeability and tissue homeostasis.
• PLVAP is widely expressed in tissues with fenestrated endothelium, including lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, and liver, as well as in tumor-associated endothelium.
• Subcellularly, PLVAP localizes to the cell membrane, caveolae, and perinuclear region, consistent with its role in vesicular trafficking and membrane microdomain organization.
• The protein contains coiled-coil regions and a signal-anchor sequence, and it is predicted to have a single transmembrane helix.
• Keywords from UniProt include "Disease variant," indicating that mutations in PLVAP may be associated with pathological conditions, though specific variants are not detailed in this record.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 63-442 of human PLVAP (NP_112600.1), covering a large extracellular and cytoplasmic portion, which may generate antibodies recognizing multiple epitopes.
• For Western blot (WB), suggested starting dilutions should be empirically determined. Positive control lysates from tissues with high PLVAP expression, such as human lung or kidney, may be used to verify the band at ~51 kDa.
• The antibody cross-reacts with mouse and rat PLVAP, making it suitable for studies in rodent models; however, users should confirm specificity in their experimental system.
• In ELISA applications, the polyclonal nature may provide strong signal but may require careful optimization of coating and detection conditions.
• As a polyclonal antibody, lot-to-lot consistency should be monitored, and users may consider performing a titration for each new lot when switching between lots in long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner, providing diagnostic manufacturers and research laboratories with a streamlined source for high-quality antibodies and reagents. For endothelial and vascular biology research, CamelBio offers validated polyclonal antibodies like this anti-PLVAP, alongside matched antibody pairs, optimized monoclonal options, and bulk ancillary reagents. Whether you need routine supply or assistance sourcing rare targets such as PLVAP, CamelBio supports your project from concept to clinical development.