Anti-PLSCR3 Rabbit Monoclonal Antibody for WB and ELISA - Q9NRY6

Core Product Specifications and Parameters

Biological Background: PLSCR3 Function and Localization

• PLSCR3 (phospholipid scramblase 3) is a Ca²⁺-dependent enzyme that catalyzes rapid, bidirectional, non-specific translocation of phospholipids across mitochondrial membranes, crucial for lipid asymmetry maintenance. Related references: PMID:14573790, PMID:17226776, PMID:18358005
• It is essential for mitochondrial respiratory function, morphology, and apoptotic signaling, with scramblase activity directly linking lipid redistribution to cell death pathways. Related references: PMID:12649167, PMID:14573790, PMID:17226776
• PLSCR3 mediates cardiolipin exposure on the mitochondrial outer membrane, which facilitates t-Bid-induced cytochrome c release and amplifies caspase activation during apoptosis. Related references: PMID:14573790, PMID:17226776, PMID:18358005
• It also regulates the biosynthesis and remodeling of cardiolipin, a mitochondrial-specific phospholipid critical for cristae structure and respiratory supercomplex assembly. Related references: PMID:16939411
• Enhances TNFSF10/TRAIL-induced apoptosis by altering cardiolipin distribution, leading to increased release of apoptogenic factors and downstream caspase activation. Related references: PMID:18491232
• Subcellularly, PLSCR3 resides primarily in the mitochondria, with localization reported at both the inner membrane and nucleus, suggesting potential non‑scramblase functions.
• Tissue profiling indicates broad expression in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, uterus, small intestine, and peripheral blood lymphocytes; expression is reportedly absent in testis and brain, while liver detection remains ambiguous in the UniProt record.
• Post‑translational modifications include palmitoylation and phosphorylation; the protein contains multiple transmembrane helices and an SH3‑binding domain, consistent with its membrane‑anchored regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 1‑100 of human PLSCR3; epitope likely resides at the N‑terminus, useful for detecting full‑length protein.
• For western blotting, the predicted band size is ~32 kDa (based on the protein weight provided); validate with appropriate positive controls such as human, mouse, or rat mitochondrial lysates.
• This rabbit monoclonal antibody offers consistent lot‑to‑lot performance; confirm optimal dilution and detection conditions experimentally.
• For ELISA, pair with a matched detection antibody after standardizing coating and incubation parameters.
• Given tissue‑specific expression, enrichment from mitochondrial fractions may improve signal in tissues where PLSCR3 abundance is low.
• Always include appropriate controls and confirm specificity, particularly in model systems where expression may be regulated by apoptotic stimuli.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement, bridging diagnostic developers and researchers to high‑quality reagents for mitochondrial biology and apoptosis research. Beyond this PLSCR3 monoclonal antibody, our one‑stop platform supplies validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting projects from concept to clinic. Partner with CamelBio to accelerate your diagnostic workflows with reliable, production‑ready materials.