Anti-PLS3 Rabbit Polyclonal Antibody for WB, IP, ELISA - P13797

Core Product Specifications and Parameters

Biological Background: PLS3 Function and Localization

• PLS3, also known as T-plastin or T-fimbrin, is an actin-bundling protein encoded by the PLS3 gene.
• It cross-links actin filaments into bundles, contributing to cytoskeletal organization and cellular architecture.
• As a member of the plastin family, PLS3 specifically belongs to the T-plastin subgroup and undergoes alternative splicing to generate multiple transcript variants.
• PLS3 is predominantly localized to the cytoplasm, where it associates with the actin cytoskeleton.
• It is widely expressed in tissues including muscle, brain, uterus, and esophagus.
• PLS3 is a phosphoprotein with calcium- and metal-binding properties; its 3D structure has been determined, and it is implicated in osteoporosis and disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–240 of human PLS3, likely targeting the N-terminal region containing the first actin-binding domain.
• For WB, consider using whole cell lysates or cytoplasmic extracts from human, mouse, or rat tissues known to express PLS3 (e.g., muscle, brain).
• For IP, validate antibody-antigen complex formation under native conditions in your system. For ELISA, use recombinant PLS3 protein as a standard to establish assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. For actin cytoskeleton and bone disease research, CamelBio can supply optimized polyclonal antibodies such as this anti-PLS3 antibody alongside complementary reagents to streamline assay development.