Anti-PLP1 Polyclonal Antibody for WB, IHC, IF, ELISA - P60201

Core Product Specifications and Parameters

Biological Background: PLP1 Function and Localization

• Myelin proteolipid protein (PLP1), also known as lipophilin, is the most abundant protein in central nervous system myelin.
• It plays an essential role in the formation and maintenance of the multilamellar structure of the myelin sheath, facilitating rapid nerve impulse conduction.
• The PLP1 gene undergoes alternative splicing, producing isoforms such as PLP and DM20, which differ in a cytoplasmic loop region.
• PLP1 localizes to the cell membrane and is highly enriched in the myelin membrane, where it interacts with lipids and other myelin proteins.
• Post-translational modifications include palmitoylation and phosphorylation; it contains disulfide bonds and multiple transmembrane helices.
• Mutations in PLP1 are associated with hereditary spastic paraplegia (SPG2) and leukodystrophy (Pelizaeus-Merzbacher disease), underscoring its critical role in myelin stability.
• The protein is a lipoprotein with a molecular weight of approximately 30 kDa, consistent with its transmembrane nature.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 90-150) corresponds to a cytoplasmic loop region of PLP1, which helps ensure specificity for the native protein.
• For Western blot, a band around 30 kDa is expected; note that PLP1 may run as multiple bands due to aggregation or post-translational modifications.
• For immunohistochemistry (IHC) and immunofluorescence (IF), antigen retrieval may be required for formalin-fixed paraffin-embedded tissues; test different retrieval methods.
• Cross-reactivity with mouse and rat is reported, but validate in the relevant sample system before quantitative studies.
• Consider using this antibody for ELISA development; pair with a monoclonal detection antibody for sandwich ELISA applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop supply of IVD raw materials, supporting diagnosticians and researchers with high-quality antibodies and ancillary reagents. For neuroscience targets like PLP1, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk reagents to streamline your myelin-related assay development. Our sourcing network also covers rare targets, helping you move from concept to clinic efficiently.